この研究は、植物の根形成の研究モデルシステムであるリョクトウ(Vignaradiata syn. Phaseolus aureus)を用いた実験で、900 MHz携帯電話電磁界(EMF)ばく露が根の形成に影響を与える生化学的メカニズムを調べた。その結果、携帯電話EMFばく露群では、対照群に比べ、リョクトウの胚軸におけるプロテアーゼ活性を1.52 - 2.33倍に、ポリフェノールオキシダーゼ活性を1.5 - 4.3倍に、ペルオキシダーゼ活性を1.5 - 2.0倍に増加させた;さらに、EMFばく露群の胚軸では、マロンジアルデヒド含有量(脂質過酸化の指標)、過酸化水素およびプロリンの含有量(活性酸素種による酸化的損傷の指標)が増加した;EMFにより酸化的損傷が生じたことは、このような損傷を保護する働きをする抗酸化酵素(スーパーオキシドジスムターゼ、アスコルビン酸ペルオキシダーゼ、グアイアコールペルオキシダーゼ、カタラーゼ、およびグルタチオン還元酵素)の活性上昇が見られことからも確認された、と報告している。
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To study the effects of 900 MHz cell phone exposure on root formation in mung bean hypocotyls (stem of a germinating seedling, found below the seed leaves and above the root), a model system for rhizogenesis in plants.
16 glass vials (each containing five hypocotyls) were used for each exposure condition (including control). Each experiment (exposure condition) was repeated for three times. After the exposure, the hypocotyls were transferred into a growth chamber for seven days. For each enzymatic/biochemical analysis, there were five replicated tissue samples.
周波数 | 900 MHz |
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タイプ |
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ばく露時間 | continuous for 0.5, 1, 2 h |
Additional information | cell phones in talk and listen mode |
Modulation type | pulsed |
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Repetition frequency | 217 Hz |
Additional information |
additional frequency: 8.34 Hz |
ばく露の発生源/構造 |
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Distance between exposed object and exposure source | 6 cm |
チャンバの詳細 | with 2 mm thick aluminum sheets shielded 47.5 cm x 27 cm x 17.5 cm chamber |
ばく露装置の詳細 | two cell phones placed along the chamber's walls; 12 glass vials containing the hypocotyls positioned between them (according to the figure in the article); constant temperature of 25 +/- 1°C inside the chamber |
Sham exposure | A sham exposure was conducted. |
The number of roots per hypocotyl and the average root length decreased significantly in response to cell phone exposure. The inhibitory effect increased with the period of exposure time. Additionally, the length of emerged roots was lesser in exposed hypocotyls.
The cell phone exposure enhanced the enzyme activities of proteases, polyphenol oxidases, and peroxidases in mung bean hypocotyls in comparison with the control. Furthermore, the electromagnetic field exposure enhanced the malondialdehyde level, the hydrogen peroxide, and proline content, indicating a reactive oxygen species-mediated oxidative damage in the hypocotyls. This was confirmed by the upregulation in the enzyme activities of all antioxidant enzymes suggesting their possible role in providing protection against mobile phone exposure-induced oxidative damage.
The authors concluded that cell phone exposure affect (inhibit) the process of rhizogenesis through biochemical alterations that manifest as oxidative damage resulting in root impairment.
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