研究のタイプ: 医学／生物学の研究 (experimental study)

[小胞体ストレスに対する低出力の60.4GHzミリ波放射の直接的影響は見られない] med./bio.

Absence of direct effect of low-power millimeter-wave radiation at 60.4 GHz on endoplasmic reticulum stress

著者: Nicolaz CN, Zhadobov M, Desmots F, Sauleau R, Thouroude D, Michel D, Le Drean Y
掲載誌: Cell Biol Toxicol 2009; 25 (5): 471-478
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この研究は、低電力のMMW電磁放射が細胞生理学に及ぼす潜在的な影響を調べた。ヒトグリア細胞株U-251 MGに、電力密度0.14 mW / cm2の60.4 GHz電磁放射ばく露を与え、小胞体（ER）ストレスに対する潜在的影響の有無を評価した。ERは、環境からの刺激に非常に敏感で、その恒常性はさまざまな病状で変化することが知られている。また、ERストレスセンサであるBiP / GRP78の発現への影響をリアルタイムPCR法により調べた。その結果、60.4 GHzへのばく露は、ERストレスで見られるタンパク質の折りたたみ不全や分泌異常を引き起こさず、XBP1またはATF6の転写因子の成熟も誘導しないことが示された；またBiP / GRP78のmRNAレベルにもMMWばく露による大きな変化が見られないことが示された、と報告している。

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研究目的（著者による）

To study the potential effects of low-power millimeter wave exposure on cellular physiology (endoplasmic reticulum stress).

詳細情報

The frequency range of 60 GHz was considered in the context of their near-future applications in wireless communication systems. The endoplasmic reticulum is an organelle sensitive to a wide variety of environmental insults and involved in a number of pathologies and it is the site of synthesis and folding of secreted proteins. Perturbations of endoplasmic reticulum functions and the resulting induced pathway involves different transmembrane proteins or transcription factors (e.g. ATF6, XBP-1) which activate specific genes, such as the chaperone BiP/GRP78.
Separated transfection complexes were used: Cells were transfected with pSEAP (secreted alkaline phosphatase) reporter plasmid as marker for endoplasmic reticulum stress. This reporter enzyme is folded into its active conformation inside the endoplasmic reticulum, and only the correctly folded proteins are secreted into the medium.
Cells were transfected with BiP promoter-reporter plasmid to study stress-involved transcription factors XBP-1 and ATF6.
As endoplasmic reticulum-stress positive control, cells were treated for with thapsigargin or brefeldin or dimethyl sulfoxide.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 60.43 GHz
Modulation type: CW
ばく露時間: continuous for 24 h, 48 h or 72 h

ばく露1

主たる特性
周波数 60.43 GHz
タイプ
  • electromagnetic field
偏波
  • linear
ばく露時間 continuous for 24 h, 48 h or 72 h
Modulation
Modulation type CW
ばく露装置
ばく露の発生源／構造
ばく露装置の詳細 rectangular waveguide and pyramidal horn antenna located inside an incubator; culture plates placed in the upper part of the incubator above the antenna
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
電力密度 0.14 mW/cm² - - - -

Reference articles

  • Zhadobov M et al. (2007): [低電力60 GHzばく露はシャペロンプロテインのストレス感受性遺伝子発現を変化させない]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

Exposure to 60.4 GHz did not modify endoplasmic reticulum protein folding and secretion, nor induced XBP1 or ATF6 transcription factors maturation. There was no significant changes in gene expression of BiP/GRP78 and HSP70.
The data show that endoplasmic reticulum homeostasis does not undergo any modification at molecular level after exposure to low-power millimeter wave exposure at 60.4 GHz.

研究の種別:

研究助成

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