この研究は、GSM 1800標準の電磁界ばく露により、インビトロで作成された血液脳関門（BBB）モデルを通過するスクロースの透過性が変化するか否かを調べた。著者らの先行研究は、ばく露による透過性亢進を報告したが、その時の実験で使用したBBBの細胞培養モデルは、ブタ脳の微小血管内皮細胞（PBEC）と共培養したラット星状細胞で構成されていた。今回の実験では、細胞培養条件の最適化を行って、バリア緊密性が明らかに向上した新しいBBBモデルを使用した。このBBBモデルの細胞培養物を、先行研究と同一のばく露システムで0.3 W / kgのSARで1〜5日間ばく露を実施した。その結果、細胞層を横断するスクロースの透過は、GSM 1800電磁界への最大5日間のばく露による影響を受けなかった；したがって、先行研究で報告したようなインビトロでのEMFばく露によるBBB透過性亢進は確認できなかった；今回の実験に用いたインビトロのBBBモデルの緊密性は、インビボの状態により近いものになっている、と報告している。
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The authors previously reported that electromagnetic fields (GSM 1800 standard, 1800 MHz) increased sucrose permeation across the blood-brain barrier in vitro (see publication 3911).
In this study the cell culture model was optimized (the filter inserts were changed) in a way that it now displays barrier properties that come very close to the low in vivo permeability.
Three different blood-brain barrier in vitro models were used. These models were comprised of 1) co-cultures of porcine brain capillary endothelial cells and rat astrocytes, 2) solitary porcine brain capillary endothelial cells cultivated in medium with addition of serum, and 3) without serum but with hydrocortisone.
| 周波数 | 1.8 GHz |
|---|---|
| タイプ |
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| 特性 |
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| ばく露時間 | continuous for up to 5 days |
| Modulation type | pulsed |
|---|---|
| Pulse width | 0.577 ms |
| Repetition frequency | 217 Hz |
| ばく露の発生源／構造 |
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|---|---|
| ばく露装置の詳細 | 4-well dishes containing the cell cultures were placed into a waveguide for exposure and into another waveguide for sham exposure. The arrangement of the cells inside the waveguides was exactly as described in the reference article. Small holes were drilled in the waveguides to allow for the equilibration of the atmosphere required for the cell cultures. Both of these waveguides were located in an incubator. A control group of cell cultures was placed in the same incubator but outside the waveguides. |
| Additional information | Since the waveguides were not designed to include a temperature probe, temperature control of the exposed cells was not possible. |
In all three blood-brain barrier models, sucrose permeation across the cell layers remained unaffected by exposure to a 1800 MHz field for up to 5 days.The authors thus could not confirm enhanced permeability of the blood-brain barrier in vitro after electromagnetic field exposure as reported before since the in vitro barrier tightness in these experiments is now more like that of the in vivo situation.
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