The authors previously reported that electromagnetic fields (GSM 1800 standard, 1800 MHz) increased sucrose permeation across the blood-brain barrier in vitro (see publication 3911).
In this study the cell culture model was optimized (the filter inserts were changed) in a way that it now displays barrier properties that come very close to the low in vivo permeability.
Three different blood-brain barrier in vitro models were used. These models were comprised of 1) co-cultures of porcine brain capillary endothelial cells and rat astrocytes, 2) solitary porcine brain capillary endothelial cells cultivated in medium with addition of serum, and 3) without serum but with hydrocortisone.
| Frequency | 1.8 GHz |
|---|---|
| Type | |
| Charakteristic |
|
| Exposure duration | continuous for up to 5 days |
| Modulation type | pulsed |
|---|---|
| Pulse width | 0.577 ms |
| Repetition frequency | 217 Hz |
| Exposure source |
|
|---|---|
| Setup | 4-well dishes containing the cell cultures were placed into a waveguide for exposure and into another waveguide for sham exposure. The arrangement of the cells inside the waveguides was exactly as described in the reference article. Small holes were drilled in the waveguides to allow for the equilibration of the atmosphere required for the cell cultures. Both of these waveguides were located in an incubator. A control group of cell cultures was placed in the same incubator but outside the waveguides. |
| Additional info | Since the waveguides were not designed to include a temperature probe, temperature control of the exposed cells was not possible. |
In all three blood-brain barrier models, sucrose permeation across the cell layers remained unaffected by exposure to a 1800 MHz field for up to 5 days.The authors thus could not confirm enhanced permeability of the blood-brain barrier in vitro after electromagnetic field exposure as reported before since the in vitro barrier tightness in these experiments is now more like that of the in vivo situation.
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