研究のタイプ: 医学／生物学の研究 (experimental study)

[環境非電離放射による細胞タンパクの変化. 1. 熱ショックタンパク] med./bio.

Changes in cellular proteins due to environmental non-ionizing radiation. I. Heat-shock proteins

著者: Kwee S, Raskmark P, Velizarov S
掲載誌: Electro Magnetobiol 2001; 20 (2): 141-152
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この研究は、さまざまな温度で培養された細胞における熱ショックタンパク質の発現量に対する弱いマイクロ波電磁界の影響を調べた。実験に用いた電磁界は、携帯電話で使用される960 MHz GSM信号をシミュレートしたものである。TEMセル内で、ガラス製カバースリップ上で増殖中の形質転換ヒト上皮羊膜（AMA）細胞に電磁界ばく露を与えた。細胞内でのSARは2.1 mW/ kgと推定された。ばく露時の培養温度はそれぞれ35、37、および40±0.1°Cに設定し、ばく露時間は20分とした。熱ショックタンパク質Hsp-70およびHsp-27を免疫蛍光法により測定した。その結果、35および37°Cでばく露した細胞には、擬似ばく露細胞に比べて多くのHsp-70が存在した；ばく露した電磁界強度は、マイクロ波による加熱効果がないとされる安全基準値よりはるかに低いレベルであるので、観察された影響は非熱的なものであると考えられる；Hsp-27に関しては有意な影響は見られなかった、と報告している。

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研究目的（著者による）

To study the effect of weak microwave fields on the amounts of heat-shock proteins in cell cultures at various temperatures (35, 37, and 40°C). To study the effect of heating only, the cells were placed in the field-free exposure incubator and were heated to the same and also to higher temperatures up to 42°C.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 960 MHz
Modulation type: pulsed
ばく露時間: continuous for 20 min

ばく露1

主たる特性
周波数 960 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 20 min
Modulation
Modulation type pulsed
Duty cycle 12 %
Repetition frequency 217 Hz
Pulse type rectangular
Additional information

simulated GSM
ばく露装置
ばく露の発生源／構造
チャンバの詳細 A TEM cell of 320 mm length and 160 mm TEM region was located in an incubator maintained at 35, 37, and 40 ± 0.1°C.
ばく露装置の詳細 A Petri dish containing 4 glass cover slips with cell cultures was placed in the TEM cell. A control dish was placed outside the TEM cell at a distance of 20 cm from the exposed dish.
Additional information Sham experiments were made with the TEM cell not powered. Effects of heating only (up to 42°C) were studied by placing the coverslips in a field-free incubator.
パラメータ
測定量 値 種別 Method Mass 備考
電界強度 17 mV/cm peak 計算値 - -
SAR 2.1 mW/kg unspecified 計算値 - -
Additional parameter details

Static and 50 Hz AC MF measured at TEM cell position were 1 µT.

測定および計算の詳細

For calculation of the SAR, the same method was applied as in previous experiments (see reference article). The SAR value was obtained from FDTD calculations of the EMF distribution in a microtiter suspension well. The dielectric parameters were based on the composition of a mammalian cell also assumed for cells growing on coverslips.

Reference articles

  • Kwee S et al. (1998): [環境中の非電離放射線による細胞増殖の変化 2.マイクロ波放射]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

At both 35 and 37°C, higher amounts of Hsp-70 were found in the exposed cells as compared with the sham-exposed cells. The effects can be considered to be athermal. In the heat control experiments the cells had to be heated up to 3-5°C higher, or for much longer time, to get the same maximum responses as were induced by the microwave irradiation. There was no significant response in the case of Hsp-27.

研究の種別:

研究助成

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