The effects of exposure of human lung fibroblasts to a 50 Hz magnetic field on proliferation and oxidative homeostasis should be investigated.
Cells were divided into the following groups: 1) exposure for 1 day, 2) exposure for 2 days, 3) exposure for 3 days, 4) exposure for 7 days and 5) control group.
Cells were examined 24 hours after the end of exposure, respectively.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
50 Hz
Exposure duration:
1 hour/day for 1, 2, 3 or 7 days
|
|
| Frequency | 50 Hz |
|---|---|
| Type | |
| Exposure duration | 1 hour/day for 1, 2, 3 or 7 days |
| Exposure source | |
|---|---|
| Chamber | cell culture flask and 96-well plates |
| Setup | solenoid (60 cm diameter) placed in a incubator; cells were placed in the central area of the solenoid where the magnetic field was most uniform |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 10 mT | - | - | - | - |
Cell proliferation was significantly increased after 2 and 7 days of exposure (groups 2 and 4) and significantly decreased after 3 days of exposure (group 3) compared to the control group. Several significant differences were found in parameters of oxidative homeostasis between exposure groups and the control group. The authors interpreted the results as follows: exposure to a magnetic field for more than 2 days exceeds the defense capacity of the cells antioxidative system, which in turn results in disruption of the cell cycle. This disruption is reflected by an increased cell proliferation induced by peroxynitrite accumulation (groups 2 and 4) and in the reduction of cell proliferation due to accumulation of cytotoxic oxidized glutathione (group 3).
The authors concluded that exposure of human lung fibroblasts to a 50 Hz magnetic field might affect proliferation and oxidative homeostasis depending on the exposure duration.
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