The effects of exposure of human adipose-derived mesenchymal stem cells cells to a 50 Hz magnetic field on cell proliferation should be investigated.
The cells were divided into the following groups: 1) exposure to a 0.5 mT magnetic field for 20 minutes/day, 2) exposure to a 0.5 mT magnetic field for 40 minutes/day, 3) exposure to a 1 mT magnetic field for 20 minutes/day, 4) exposure to a 1 mT magnetic field for 40 minutes/day, 5) sham exposure for 20 minutes/day, 6) sham exposure for 40 minutes/day.
All experiments were repeated three times.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
50 Hz
Exposure duration:
20 to 40 minutes/day for 7 days
|
|
|
Exposure 2:
50 Hz
Exposure duration:
20 to 40 minutes/day for 7 days
|
|
| Frequency | 50 Hz |
|---|---|
| Type | |
| Waveform | |
| Exposure duration | 20 to 40 minutes/day for 7 days |
| Exposure source | |
|---|---|
| Chamber | 96-well plates |
| Setup | solenoid was made of 720 turns of 1 mm enamel copper wire on a cylindrical core of acrylic tube (inner diameter: 20 cm, height: 24 cm); the uniformity of the magnetic field at the center of the solenoid, where the cell cultures were located, was ±1%; the temperature in solenoid during exposure was controlled by a digital thermometer and magnetic field-induced heating was negligible |
| Sham exposure | A sham exposure was conducted. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 0.5 mT | - | measured | - | - |
| Frequency | 50 Hz |
|---|---|
| Type | |
| Waveform | |
| Exposure duration | 20 to 40 minutes/day for 7 days |
| Exposure source |
|
|---|---|
| Sham exposure | A sham exposure was conducted. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 1 mT | - | measured | - | - |
In the trypan blue dye exclusion test, cell viability and cell proliferation were significantly increased in all exposure groups (groups 1-4) compared to sham exposure groups (groups 5 and 6). In the MTT test, cell viability and cell proliferation were significantly increased in all exposure groups except for group 4 (1 mT for 40 min/day) compared to the sham exposure groups. No differences in cell morphology were found between the exposure and sham exposure groups.
The authors conclude that exposure of human adipose-derived mesenchymal stem cells cells to a 50 Hz magnetic field might promote cell proliferation.
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