The effects of exposure of dental pulp stem cells to 50 Hz magnetic fields on cell viability and genotoxicity should be investigated.
The study background was a possible use in regenerative endodontic procedures.
Cells were divided into the following groups: exposure to a magnetic field of 1) 0.5 mT for 20 min/day, 2) 0.5 mT for 40 min/day, 3) 1 mT for 20 min/day, 4) 1 mT for 40 min/day, 5) control group for 20 min/day exposure, 6) control group fpr 40 min/day exposure. All cells were investigated after 7 days of exposure.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
50 Hz
Exposure duration:
20 min/day or 40 min/day for 7 days
|
|
|
Exposure 2:
50 Hz
Exposure duration:
20 min/day or 40 min/day for 7 days
|
|
| Frequency | 50 Hz |
|---|---|
| Waveform | |
| Exposure duration | 20 min/day or 40 min/day for 7 days |
| Exposure source | |
|---|---|
| Setup | The solenoid was wound with 720 turns of 1 mm enamel copper wire on a cylindrical core of acrylic tube (inner diameter: 20 cm, height: 24 cm); the uniformity of the magnetic field was ± 1% at the centre of the solenoid where the cell cultures were located |
| Sham exposure | A sham exposure was conducted. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 0.5 mT | - | calibration | - | - |
| Frequency | 50 Hz |
|---|---|
| Waveform | |
| Exposure duration | 20 min/day or 40 min/day for 7 days |
| Exposure source |
|
|---|---|
| Sham exposure | A sham exposure was conducted. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 1 mT | - | calibration | - | - |
In all exposure groups (groups 1-4), cells extended on the surfaces of the culture vessels as the fibroblastic cells in contrast to the control groups. Cell viability was significantly increased in all exposure groups except for group 4 (1 mT for 40 min/day) compared to the control groups, with group 3 (1 mT for 20 min/day) showing the largest increase. Genotoxicity was at least signficantly increased in groups 2 (0,5 mT for 40 min) and 3 compared to the respective control group (remark EMF-Portal: the results for the other groups are presented ambiguously).
The authors conclude that exposure of dental pulp stem cells to 50 Hz magnetic fields might increase the cell viability.
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