To investigate the effects of extremely low frequency magnetic fields on the proliferation and cell viability of human adipose derived stem cells.
Cells were exposed to magnetic field intensities of 0.5 mT or 1 mT. Exposure duration was 20 or 40 minutes per day.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
50 Hz
Exposure duration:
20 or 40 minutes per day for 7 days
|
|
|
Exposure 2:
50 Hz
Exposure duration:
20 or 40 minutes per day for 7 days
|
|
| Frequency | 50 Hz |
|---|---|
| Type | |
| Waveform | |
| Exposure duration | 20 or 40 minutes per day for 7 days |
| Exposure source | |
|---|---|
| Chamber | cells were cultured and exposed in 96 well plates |
| Setup | solenoid consisted of 720 turns of 1 mm enamel copper wire and was wound on a cylindrical core of acrylic tube (inner diameter 20 cm, height 24 cm); solenoid was connected to an autotransformer which was connected to 220 V power; uniformity of magnetic field at the center of solenoid was ± 1% where cultures were located |
| Sham exposure | A sham exposure was conducted. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 0.5 mT | - | measured | - | - |
| Frequency | 50 Hz |
|---|---|
| Type | |
| Waveform | |
| Exposure duration | 20 or 40 minutes per day for 7 days |
| Exposure source |
|
|---|
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 1 mT | - | measured | - | - |
The cell proliferation and cell viability were significantly increased in cell cultures exposed to a magnetic field intensity of 1 mT for 20 minutes and of 0.5 mT for 20 and 40 minutes compared to the sham exposure.
The authors conclude that exposure to extremely low frequency magnetic fields could enhance the cell viability and cell proliferation of human adipose derived stem cells.
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