研究のタイプ: 医学／生物学の研究 (experimental study, theoretical study)

[低パルス周波、高周波電磁界はカタツムリの神経細胞の基本的なパラメータに影響するか　I.　電磁界] med./bio.

Do weak, low pulsed frequency, high-frequency electromagnetic or magnetic fields alter the basic bioelectrical parameters of nerve cells in vineyard snails (Helix pomatia L.) ? / I. Electromagnetic fields

著者: Kullnick U
掲載誌: Bioelectrochem Bioenerg 1995; 37 (1): 39-45
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この研究は、リンゴマイマイ（食用カタツムリ、学名ヘリックス・ポマティア）の安静時電位（RP）への低レベルの低周波パルス変調高周波電磁界の影響を調べた。ばく露の強度は、組織に熱的影響が生じ得ないレベルを選択した。その結果、8.3Hzパルス変調150MHz電磁界、磁束密度124μTで、非熱的影響（神経細胞でのRPの過分極）が観察された；ばく露で誘導された過分極の大きさとばく露前のRPとの間の関係が確立された；電磁界ばく露による過分極誘導は、細胞内からのカルシウム流出が関与する仮説的モデルで説明される、と報告している。

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研究目的（著者による）

To investigate resting potentials of the vineyard snail (Helix pomatia) to find how they were influenced by weak, low pulsed frequency, high-frequency electromagnetic fields. The flux densities of the fields were chosen such that thermal effects could not occur in the tissue exposed. An experimental method was developed which allowed electrophysiological measurements to be carried out in the high-frequency electromagnetic field without artifacts.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 150 MHz
Modulation type: pulsed
ばく露時間: continuous for 10 min
ばく露2: 150 MHz
Modulation type: pulsed
ばく露時間: continuous for 20 min or intermittent for 2 x 10 min
ばく露3: 150 MHz
Modulation type: pulsed
ばく露時間: continuous for 10 min

ばく露1

主たる特性
周波数 150 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 10 min
Modulation
Modulation type pulsed
Repetition frequency 8.3 Hz
ばく露装置
ばく露の発生源／構造
  • therapy equipment Megawave 150/1
Distance between exposed object and exposure source 2 cm
チャンバの詳細 A small Plexiglas vessel was attached to a vibration-damped marble slab in a Faraday cage.
ばく露装置の詳細 The transmitting antenna was fastened 2 cm away from the ganglion.
パラメータ
測定量 値 種別 Method Mass 備考
磁束密度 50 µT - - - ± 50%
磁束密度 70 µT - - - ± 50%
磁束密度 90 µT - - - ± 50%
磁束密度 124 µT - - - ± 50%

ばく露2

主たる特性
周波数 150 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 20 min or intermittent for 2 x 10 min
Modulation
Modulation type pulsed
Repetition frequency 8.3 Hz
ばく露装置
ばく露の発生源／構造
  • E1と同じ装置
Additional information The run down of 40 min served as a run up for the second exposure.
パラメータ
測定量 値 種別 Method Mass 備考
磁束密度 124 µT - - - ± 50%
電力密度 0.02 mW/cm² - - - ± 50%

ばく露3

主たる特性
周波数 150 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 10 min
Modulation
Modulation type pulsed
Additional information

pulse rates of 6, 10, 12, and 50 Hz
ばく露装置
ばく露の発生源／構造
  • E1と同じ装置
パラメータ
測定量 値 種別 Method Mass 備考
磁束密度 124 µT - - - ± 50%

Reference articles

  • Kullnick U (1992): [弱非熱的高周波数電磁界が神経細胞の膜電位に与える影響]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム（著者による）

The resting potential of the neurons being investigated hyperpolarized. A relationship between the resulting value of the strength of the field-induced hyperpolarization and the resting potential before the start of the exposure was established. A model of the sequence of events for the effect was developed in order to explain the exposure-induced hyperpolarization. Fundamental to the hypothesis which was drawn up is the interaction of a possible field-induced release of calcium from intracellular depots in the cytoplasma of neurons and the resultant activation of the calcium-dependent potassium channels.

研究の種別:

研究助成

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