研究のタイプ: 医学/生物学の研究 (experimental study)

[PC12細胞の増殖およびアポトーシスに対する50Hz磁界と磁性ナノ粒子の複合影響] med./bio.

Combined effects of 50 Hz magnetic field and magnetic nanoparticles on the proliferation and apoptosis of PC12 cells

掲載誌: Biomed Environ Sci 2014; 27 (2): 97-105

この研究は、PC12細胞に対する超低周波磁界MF;50Hz、400 μT)および磁気ナノ粒子(MNPs)の影響を、細胞毒性試験およびアポトーシス試験により調べた。シリカゲル被覆MNPs(MNP-SiO2;直径20 nm程度;強磁性))投与の有無、48時間のMFばく露の有無の組合せによる4群で実験し、MTT試験により細胞毒性を、アネキシンV-FITC/PI染色法によりアポトーシスを評価した。また、透過電子顕微鏡により形態学とMNP-SiO2の摂取状況を観察した。その結果、MNP-SiO2は、量依存的に細胞生存率を減少させた;MFは、MNP-SiO2の濃度の増加につれて細胞生存率を低下させた;MNP-SiO2単独ばく露ではアポトーシスを生じなかった;一方、MFばく露下ではMNP-SiO2の濃度の増加につれてアポトーシス細胞の割合が増加した;MNP-SiO2は細胞に取り込まれ、細胞の形態を若干変化させた、と報告している。

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研究目的(著者による)

To study the effects of extremely low frequency magnetic fields and magnetic nanoparticles via cytotoxicity and apoptosis assays on PC12 cells.

詳細情報

PC12 cells were treated with magnetic nanoparticles (MNP-SiO2; 0, 20 and 100 µg/ml) with or without magnetic field exposure for 48 h.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 48 h

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 48 h
ばく露装置
ばく露の発生源/構造
チャンバの詳細 cells were seeded in plates with 24 or 6 wells; plates were placed in a 5% CO2 incubator at 37°C
ばく露装置の詳細 double-wrapped coil system consisting of an alternating power source and two solenoid coils; each solenoid coil was composed of a core cylindrical tube of epoxy resins (inner diameter 200 mm, length 350 mm) and two subcoils with 160 turns each of enamel copper wire (diameter 2.06 mm); solenoid coils were placed into two CO2 incubators; direction of the magnetic field was vertical
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 400 µT effective value - - -

Reference articles

  • Kirschvink JL (1992): [均一磁界とダブル巻きコイルシステム:生体電磁気実験のための設計改善技術]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The magnetic nanoparticles reduced the cell viability in a dose-dependent manner. The magnetic field exposure also reduced the cell viability with increasing concentrations of the magnetic nanoparticles. The magnetic nanoparticles alone did not cause apoptosis in PC12 cells; however, the proportion of apoptotic cells increased significantly under magnetic field exposure and increasing doses of nanoparticles. The magnetic nanoparticles could be ingested and then cause a slight change in cell morphology.
The authors conclude that combined exposure of magnetic fields and magnetic nanoparticles resulted in remarkable cytotoxicity and increased apoptosis in PC12 cells. The results suggest that magnetic field exposure could strengthen the magnetic field of magnetic nanoparticles, which may enhance the bioeffects of extremely low frequency magnetic fields.

研究の種別:

研究助成

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