この研究は、発達中の大脳皮質での総タンパク濃度（TPC）、リーリンおよびDab1発現に対する磁界の影響を調べた。神経上皮で作られた神経細胞は放射状グリア線維に沿って皮質板に移動するが、カハールレチウス細胞が分泌するリーリンはこの移動に必須であり、大脳皮質の発生に重要な役割を果たす。30匹の妊娠したBalb/cマウスを10匹ずつの対照群、磁界ばく露群、擬似磁界ばく露群に分けた。妊娠15日目のマウスをソレノイドコイル内に置き、約1 mT（50 Hz ）に1日5時間、3日間連続でばく露した（15-17日目）。ばく露終了から24時間後に摘出した胎仔156匹（ばく露群56、擬似ばく露群48、対照群42）の大脳皮質組織標本のTPC、リーリンおよびDab1発現を測定した。その結果、ばく露群のTPCは、擬似ばく露群・対照群に比べ、有意に変化しなかった；ばく露群のリーリンおよびDab1発現は、擬似ばく露群・対照群に比べ、増加した、と報告している。
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To investigate the effects of exposure to a 50 Hz-magnetic field on total protein concentration as well as protein expression of reelin and Dab1 in the developing cortex of mice.
Reelin is a protein which helps to regulate processes of neural migration and positioning in the developing brain via cell-cell interactions. Disabled-1 (Dab1) is an important regulator of Reelin signalling.
Pregnant mice were divided into three groups (n=10 per group): 1.) cage control (embryos n=42), 2.) sham exposure group (embryos n=48) and 3.) exposure group (embryos n= 56). Exposure or sham exposure took place from gestational day 15-17. Dams were killed on gestational day 18 and cortices of embryos were collected for examinations.
| ばく露 | パラメータ |
|---|---|
|
ばく露1:
50 Hz
ばく露時間:
5 hours/day for 3 consecutive days
|
|
| 周波数 | 50 Hz |
|---|---|
| タイプ |
|
| ばく露時間 | 5 hours/day for 3 consecutive days |
| ばく露の発生源／構造 | |
|---|---|
| ばく露装置の詳細 | urban electric line adaptor 220 V and 10 A was used for minimizing the heat production, EMF emitter set including bobbin (80 x 10), wires and metal nucleuses was put in the bottom of hatchery machine in a metal lacuna, calibration of the exposure facility was performed to confirm uniform distribution of the magnetic field intensity where mice were kept, temperature and humidity were monitored continuously |
| Sham exposure | A sham exposure was conducted. |
| 測定量 | 値 | 種別 | Method | Mass | 備考 |
|---|---|---|---|---|---|
| 磁束密度 | 0.5 mT | - | - | - | - |
No significant differences between the groups were seen regarding the total protein concentration. Additionally, no significant differences in the protein expression were seen between the cage control and the sham exposed group. However, in cortices from the exposure group the protein expression of Reelin and Dab1 was significantly increased compared to the cage control and the sham exposed group.
The authors conclude that exposure to a 50 Hz-magnetic field could influence the protein expression of Reelin and Dab1 in the developing cortex of mice.
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