この研究は、同調チャイニーズハムスター卵巣細胞株（CHO）に、等温（37±0.2℃）条件下で、連続波の27MHzの無線周波（RFR）または2450MHzマイクロ波（MWR）の2時間ばく露または擬似ばく露を行い、1）高周波電磁界が熱影響ではなく、直接的に細胞周期に影響するか否か、2）その影響に周波数依存性があるか否か､を調べた。細胞周期のG0/G1、S、G2/Mの各相の細胞に、それぞれの周波数で、SARは5または25 W/ kgでばく露（擬似ばく露）を行い、その後4日間、フローサイトメトリで細胞周期の変化およびDNA量を観察した。その結果、両方の周波数、両方のSARレベルの各ばく露群で、ばく露後4日間、細胞周期に変化が見られた；27MHz群では、2450MHz群に比べ、一時的変化、細胞周期の各相の感受性、周期の全体的変化の大きさに有意差があった､と報告している。
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Synchronized cells were exposed to continuous wave (CW) 2.45 GHz microwave irradiation or CW 27 MHz radiofrequency irradiation under isothermal conditions (37 ± 0.2 °C) to test the following hypotheses: (1) high frequency electromagnetic irradiation directly affects the mammalian cell cycle in the absence of irradiation-induced heating; and (2) the magnitude of the cell cycle alteration is frequency dependent.
Cell suspensions were simultaneously exposed to 2.45 and 27 MHz RF radiation at SARs of 0, 5, or 25 W/kg.
| 周波数 | 2.45 GHz |
|---|---|
| タイプ |
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| 特性 |
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| ばく露時間 | continuous for 2 h |
| Modulation type | CW |
|---|
| ばく露の発生源／構造 | |
|---|---|
| チャンバの詳細 | Temperature was maintained at 37°C by coolant circulation from controlled baths. |
| Sham exposure | A sham exposure was conducted. |
| 周波数 | 27 MHz |
|---|---|
| タイプ |
|
| 特性 |
|
| ばく露時間 | continuous for 2 h |
| Modulation type | CW |
|---|
| ばく露の発生源／構造 | |
|---|---|
| ばく露装置の詳細 | The coaxial transmission line tapered up to inner and outer stainless steel conductors of 3.34 and 3.84 cm radius provided an annular exposure chamber. The use of this system, including dosimetric methods, has been described by Cleary et al. [1985] and Liu and Cleary [1988]. |
| Sham exposure | A sham exposure was conducted. |
Exposure to 27 MHz radiofrequency irradiation or 2.45 GHz microwave irradiation altered the cell cycle for periods of up to 4 days following exposure at SARs of 5 or 25 W/kg. There were significant differences in temporal responses, cell cycle phase sensitivity, and overall degree of cell cycle alteration for 27 MHz in comparison to 2.45 GHz irradiation. In contrast to the effect of 27 MHz radiofrequency irradiation, which did not affect G2/M-phase cells, 2.45 GHz microwave irradiation altered all cell cycle phases to varying degrees. Exposure to 2.45 GHz at 5 or 25 W/kg was twice as effective as 27 MHz irradiation in inducing cell cycle alterations as determined by differences in the number of exposed versus sham-exposed cells in various cell cycle phases.
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