非電離放射の生理学的影響は取るに足りないと考えられてきたが、本論文では、ある分子マーカー(ストレスに関するbZIP転写要因を解読するmRNA)における変化を計測するために、環境中にある電磁界の特性(等方性と均一性)を模擬する、注意深く調整された刺激システムを使用して、低振幅、短期間、900MHz, EMFがmRNAの蓄積を誘発することを示している。この蓄積は素早く(ピークは刺激後5-15分後)で強く(3.5倍)、機械刺激で起こされた蓄積に似ている。
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To study the effect of mobile phone intensity microwave irradiation on tomato plant studying the accumulation of a stress-related transcript that responds very rapidly to even small environmental stimulations.
Leaf tissue was collected at various times (0, 5, 15, 30, 60 min.) after the end of exposure.
周波数 | 900 MHz |
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タイプ |
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ばく露時間 | continuous for 10 min |
ばく露の発生源/構造 |
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チャンバの詳細 | The MSRC (mode stirred reverberation chamber) consisted of a large room (200 m³) with a double-layered metal wall, acting as a Faraday cage, a log-periodic antenna, and a rotary stirrer creating different patterns of multiple reflections on the chamber walls, thus randomizing the polarization of the electromagnetic waves. Tomato plants were grown inside EMF-permeable culture chambers. Shielded chambers were enclosed in a polymer mesh covered with an aluminium layer causing 45 dB signal attenuation to protect the plants from exposure. |
ばく露装置の詳細 | When the plants were 3 weeks old, the whole culture chamber was placed in the MSRC (18 h prior to irradiation) in a defined working volume where the field was statistically isotropic and homogeneous. |
Additional information | The fourth terminal leaf (exposed and shielded) was collected at various times after exposure (0, 5, 15, 30, and 60 min). Control plants were collected before stimulation. |
The data showed that low amplitude, short duration, 900 MHz electromagnetic field evoked the accumulation of the stress-related transcript (mRNA). This accumulation was rapid (peaking 5-15 min after exposure), strong (3.5-fold), and was similar to that evoked by injurious stimuli.
In some experiments, the accumulation began immediately after the end of the exposure, while in others there was a short delay. In all cases, the response was maximal at 5-15 min. after the end of exposure, in some cases declined somewhat at 30 min., but generally remained at high levels until 60 min.
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