研究のタイプ: 医学/生物学の研究 (experimental study)

[マイクロ波照射は植物の遺伝子発現に影響する] med./bio.

Microwave irradiation affects gene expression in plants

掲載誌: Plant Signal Behav 2006; 1 (2): 67-70

電離放射生理学的影響は取るに足りないと考えられてきたが、本論文では、ある分子マーカー(ストレスに関するbZIP転写要因を解読するmRNA)における変化を計測するために、環境中にある電磁界の特性(等方性と均一性)を模擬する、注意深く調整された刺激システムを使用して、低振幅、短期間、900MHz, EMFがmRNAの蓄積を誘発することを示している。この蓄積は素早く(ピークは刺激後5-15分後)で強く(3.5倍)、機械刺激で起こされた蓄積に似ている。

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研究目的(著者による)

To study the effect of mobile phone intensity microwave irradiation on tomato plant studying the accumulation of a stress-related transcript that responds very rapidly to even small environmental stimulations.

詳細情報

Leaf tissue was collected at various times (0, 5, 15, 30, 60 min.) after the end of exposure.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 900 MHz
ばく露時間: continuous for 10 min

ばく露1

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 10 min
ばく露装置
ばく露の発生源/構造
  • MSRC
チャンバの詳細 The MSRC (mode stirred reverberation chamber) consisted of a large room (200 m³) with a double-layered metal wall, acting as a Faraday cage, a log-periodic antenna, and a rotary stirrer creating different patterns of multiple reflections on the chamber walls, thus randomizing the polarization of the electromagnetic waves. Tomato plants were grown inside EMF-permeable culture chambers. Shielded chambers were enclosed in a polymer mesh covered with an aluminium layer causing 45 dB signal attenuation to protect the plants from exposure.
ばく露装置の詳細 When the plants were 3 weeks old, the whole culture chamber was placed in the MSRC (18 h prior to irradiation) in a defined working volume where the field was statistically isotropic and homogeneous.
Additional information The fourth terminal leaf (exposed and shielded) was collected at various times after exposure (0, 5, 15, 30, and 60 min). Control plants were collected before stimulation.
パラメータ
測定量 種別 Method Mass 備考
電界強度 5 V/m - - - input amplitude
電界強度 3.9 V/m - - - inside the culture chamber
電界強度 0.5 V/m - - - shielded culture chamber

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露前
  • ばく露後

研究の主なアウトカム(著者による)

The data showed that low amplitude, short duration, 900 MHz electromagnetic field evoked the accumulation of the stress-related transcript (mRNA). This accumulation was rapid (peaking 5-15 min after exposure), strong (3.5-fold), and was similar to that evoked by injurious stimuli.
In some experiments, the accumulation began immediately after the end of the exposure, while in others there was a short delay. In all cases, the response was maximal at 5-15 min. after the end of exposure, in some cases declined somewhat at 30 min., but generally remained at high levels until 60 min.

研究の種別:

研究助成

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