To investigate brain tumor development in rats exposed to 915 MHz electromagnetic fields (EMF) by continuous waves (CW) and different pulse-modulated waves. A rat in vivo glioma-model, using RG2 and N32 cells, were used for tumor induction.
Exposure | Parameters |
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Exposure 1:
915 MHz
Modulation type:
CW
Exposure duration:
repeated daily exposure, 7 h/day, 5 days/week, for 10-15 days
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Exposure 2:
915 MHz
Modulation type:
pulsed
Exposure duration:
repeated daily exposure, 7 h/day, 5 days/week, for 10 days
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|
Exposure 3:
915 MHz
Modulation type:
pulsed
Exposure duration:
repeated daily exposure, 7 h/day, 5 days/week, for 10 days
|
|
Exposure 4:
915 MHz
Modulation type:
pulsed
Exposure duration:
repeated daily exposure, 7 h/day, 5 days/week, for 13 days
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|
Exposure 5:
915 MHz
Modulation type:
pulsed
Exposure duration:
repeated daily exposure, 7 h/day, 5 days/week, for 9 days
|
|
Exposure 6:
915 MHz
Modulation type:
pulsed
Exposure duration:
repeated daily exposure, 7 h/day, 5 days/week, for 9-13 days
|
Frequency | 915 MHz |
---|---|
Type | |
Charakteristic |
|
Exposure duration | repeated daily exposure, 7 h/day, 5 days/week, for 10-15 days |
Modulation type | CW |
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Exposure source | |
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Chamber | The test system consisted of four TEM cells. The TEM cell was enclosed in a well ventilated wooden box supporting the outer conductor made of brass-net and the central plate, or septum, constructed of aluminium and held up by Teflon braces. |
Setup | The animals were kept unanaesthetized and were given a half hour break for feeding after 4 hours of exposure. |
Sham exposure | A sham exposure was conducted. |
Additional info | Control animals were kept in identical TEM cells without EMF exposure. |
Frequency | 915 MHz |
---|---|
Type | |
Charakteristic |
|
Exposure duration | repeated daily exposure, 7 h/day, 5 days/week, for 10 days |
Modulation type | pulsed |
---|---|
Pulse width | 0.57 ms |
Rise time | 0.04 ms |
Fall time | 0.81 ms |
Duty cycle | 0.2 % |
Repetition frequency | 4 Hz |
Exposure source |
|
---|---|
Sham exposure | A sham exposure was conducted. |
Frequency | 915 MHz |
---|---|
Type | |
Charakteristic |
|
Exposure duration | repeated daily exposure, 7 h/day, 5 days/week, for 10 days |
Modulation type | pulsed |
---|---|
Pulse width | 0.57 ms |
Rise time | 0.04 ms |
Fall time | 0.81 ms |
Duty cycle | 0.5 % |
Repetition frequency | 8.33 Hz |
Exposure source |
|
---|---|
Sham exposure | A sham exposure was conducted. |
Frequency | 915 MHz |
---|---|
Type | |
Charakteristic |
|
Exposure duration | repeated daily exposure, 7 h/day, 5 days/week, for 13 days |
Modulation type | pulsed |
---|---|
Pulse width | 0.57 ms |
Rise time | 0.04 ms |
Fall time | 0.81 ms |
Duty cycle | 0.9 % |
Repetition frequency | 16 Hz |
Exposure source |
|
---|---|
Sham exposure | A sham exposure was conducted. |
Frequency | 915 MHz |
---|---|
Type | |
Charakteristic |
|
Exposure duration | repeated daily exposure, 7 h/day, 5 days/week, for 9 days |
Modulation type | pulsed |
---|---|
Pulse width | 0.57 ms |
Rise time | 0.04 ms |
Fall time | 0.81 ms |
Duty cycle | 12 % |
Repetition frequency | 217 Hz |
Exposure source |
|
---|---|
Sham exposure | A sham exposure was conducted. |
Frequency | 915 MHz |
---|---|
Type | |
Charakteristic |
|
Exposure duration | repeated daily exposure, 7 h/day, 5 days/week, for 9-13 days |
Modulation type | pulsed |
---|---|
Pulse width | 6 ms |
Rise time | 0.04 ms |
Fall time | 0.81 ms |
Duty cycle | 30 % |
Repetition frequency | 50 Hz |
Exposure source |
|
---|---|
Sham exposure | A sham exposure was conducted. |
In the RG2 model there was no indication of promoted tumor growth for any of the tested EMF-frequencies. As a reason for the absence of significant differences between exposed and control animals it should be kept in mind that the rat glioma tumor cell line RG2 is very aggressive, and the addition of external stimuli may therefore not influence upon the tumor growth rate. Also the less aggressive, slow-growing N32 cell line changed the tumor volume in no significant way of the exposed animals compared to their controls. In conclusion no differences were found between the growth of inoculated rat brain tumors in EMF exposed animals and their unexposed controls.
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