Normal human epidermal keratinocytes were subjected to two non-thermal exposure conditions characterized by different amplitudes and durations to study possible changes in gene expression.
Eight microarrays (four exposed and four sham exposed) were screened per electromagnetic field condition.
Exposure | Parameters |
---|---|
Exposure 1:
900 MHz
Modulation type:
CW
Exposure duration:
continuous for 10 min
|
|
Exposure 2:
900 MHz
Modulation type:
CW
Exposure duration:
continuous for 30 min
|
|
Frequency | 900 MHz |
---|---|
Type | |
Exposure duration | continuous for 10 min |
Modulation type | CW |
---|
Exposure source | |
---|---|
Setup | culture dishes individually enclosed in plastic boxes; eight boxes placed inside a temperature-controlled incubator made of 8 mm thick glas, internally insulated with 20 mm thick high-density polystyrene; incubator positioned in the working zone of a 8.4 m x 6.7 m x 3.5 m mode stirred reverberation chamber (MSRC) consisting of a metal-enclosed room; antenna pointed at a room's corner |
Sham exposure | A sham exposure was conducted. |
Measurand | Value | Type | Method | Mass | Remarks |
---|---|---|---|---|---|
electric field strength | 8 V/m | - | measured | - | - |
SAR | 2.6 mW/kg | - | calculated | - | - |
Frequency | 900 MHz |
---|---|
Type | |
Exposure duration | continuous for 30 min |
Modulation type | CW |
---|
Exposure source |
|
---|---|
Sham exposure | A sham exposure was conducted. |
Measurand | Value | Type | Method | Mass | Remarks |
---|---|---|---|---|---|
electric field strength | 41 V/m | - | measured | - | - |
SAR | 73 mW/kg | - | calculated | - | - |
Under these conditions, exposure of keratinocytes to the electromagnetic field had only a small effect: only approx. 20 genes (out of 47000) displayed a significant alteration. Four genes were up-regulated and 15 down-regulated, but none were found under both exposure conditions. In addition, ratios of expression level were calculated and the ratios were small, ranging from 0.5 (down-regulation) to 1.6 (up-regulation). Furthermore, those assayed using RT-PCR (n=15 genes) did not display a significant expression modulation (i.e. no confirmation of the microarray data).
In conclusion, the data showed that cultured keratinocytes are not significantly affected by electromagnetic field exposure.
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