To investigate whether exposure to a 50 Hz-magnetic field influences the proliferation and differentiation of embryonic neural stem cells.
Cell cultures were subjected to different magnetic flux densities (0.5, 1 or 2 mT for 3 days) and exposure times (2 mT for 1, 2 or 3 days).
Exposure | Parameters |
---|---|
Exposure 1:
50 Hz
Exposure duration:
intermittent 5 min on/10 min off for 3 days
different magnetic flux densities
|
|
Exposure 2:
50 Hz
Exposure duration:
intermittent 5 min on/10 min off for 1, 2 or 3 days
different exposure durations
|
|
Frequency | 50 Hz |
---|---|
Type | |
Waveform | |
Exposure duration | intermittent 5 min on/10 min off for 3 days |
Additional info | different magnetic flux densities |
Exposure source |
|
---|---|
Chamber | cells were exposed in plates with 96 wells (cell viability) or in 35 mm Petri dishes |
Setup | exposure system was composed of two four-coil systems (two coils with 56 windings, two coils with 50 windings) and placed inside a µ-metal chamber; both chambers were placed inside an incubator (37°C ± 0.5°C, while temperature variance between the chambers did not exceed 0.3°C; 5% CO2; 95% humidity) |
Sham exposure | A sham exposure was conducted. |
Measurand | Value | Type | Method | Mass | Remarks |
---|---|---|---|---|---|
magnetic flux density | 0.5 mT | minimum | - | - | - |
magnetic flux density | 1 mT | - | - | - | - |
magnetic flux density | 2 mT | maximum | - | - | - |
Frequency | 50 Hz |
---|---|
Type | |
Waveform | |
Exposure duration | intermittent 5 min on/10 min off for 1, 2 or 3 days |
Additional info | different exposure durations |
Exposure source |
|
---|---|
Sham exposure | A sham exposure was conducted. |
Measurand | Value | Type | Method | Mass | Remarks |
---|---|---|---|---|---|
magnetic flux density | 2 mT | - | - | - | - |
No significant differences between exposed and sham exposed cell cultures were observed regarding cell proliferation, diameter of neurospheres, cell cycle distribution, DNA synthesis, and the percentages of neurons and astrocytes. However, in exposed cell cultures, the gene expression of Math1, Math2, Ngn1 and Tuj1 was significantly increased compared to the sham exposed cell cultures, while the level of Sox2 was significantly decreased.
The authors conclude that exposure of embryonic neural stem cells to a 50 Hz-magnetic field could have an influence on the mRNA levels of genes related to neural differentiation.
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