Analysis of proto-oncogene and heat-shock protein gene expression in human derived cell-lines exposed in vitro to an intermittent 1.9 GHz pulse-modulated radiofrequency field med./bio.

Aim of study (acc. to author)

Background/further details

Endpoint

• molecular biosynthesis: gene expression

Exposure

• • mobile communications
  • digital mobile phone

Extended view Compact view

Methods Endpoint/measurement parameters/methodology

• molecular biosynthesis: gene expression (transcript levels of heat shock proteins (hsp27, hsp70) and proto-oncogenes (c-fos, c-myc, c-jun); RT-PCR)
• cell viability/cell division/proliferation: cell viability, cell number

Main outcome of study (acc. to author)

Study funded by

• Health Canada Genomics R & D fund

Related articles

• Dawe AS et al. (2008): Continuous wave and simulated GSM exposure at 1.8 W/kg and 1.8 GHz do not induce hsp16-1 heat-shock gene expression in Caenorhabditis elegans
• Chauhan V et al. (2007): Analysis of gene expression in two human-derived cell lines exposed in vitro to a 1.9 GHz pulse-modulated radiofrequency field
• Friedman J et al. (2007): Mechanism of short-term ERK activation by electromagnetic fields at mobile phone frequencies
• Zhao R et al. (2007): Studying gene expression profile of rat neuron exposed to 1800 MHz radiofrequency electromagnetic fields with cDNA microassay
• Hirose H et al. (2007): Mobile phone base station-emitted radiation does not induce phosphorylation of Hsp27
• Whitehead TD et al. (2006): Gene expression does not change significantly in C3H 10T(1/2) cells after exposure to 847.74 CDMA or 835.62 FDMA radiofrequency radiation
• Whitehead TD et al. (2006): The number of genes changing expression after chronic exposure to Code Division Multiple Access or Frequency DMA radiofrequency radiation does not exceed the false-positive rate
• Zeng Q et al. (2006): Effects of global system for mobile communications 1800 MHz radiofrequency electromagnetic fields on gene and protein expression in MCF-7 cells
• Nylund R et al. (2006): Mobile phone radiation causes changes in gene and protein expression in human endothelial cell lines and the response seems to be genome- and proteome-dependent
• Remondini D et al. (2006): Gene expression changes in human cells after exposure to mobile phone microwaves
• Lee JS et al. (2006): Radiofrequency radiation does not induce stress response in human T-lymphocytes and rat primary astrocytes
• Qutob SS et al. (2006): Microarray gene expression profiling of a human glioblastoma cell line exposed in vitro to a 1.9 GHz pulse-modulated radiofrequency field
• Chauhan V et al. (2006): Gene Expression Analysis of a Human Lymphoblastoma Cell Line Exposed In Vitro to an Intermittent 1.9 GHz Pulse-Modulated Radiofrequency Field
• Lantow M et al. (2006): Free radical release and HSP70 expression in two human immune-relevant cell lines after exposure to 1800 MHz radiofrequency radiation
• Simko M et al. (2006): Hsp70 expression and free radical release after exposure to non-thermal radio-frequency electromagnetic fields and ultrafine particles in human Mono Mac 6 cells
• Whitehead TD et al. (2005): Expression of the proto-oncogene Fos after exposure to radiofrequency radiation relevant to wireless communications
• Nikolova T et al. (2005): Electromagnetic fields affect transcript levels of apoptosis-related genes in embryonic stem cell-derived neural progenitor cells
• Laszlo A et al. (2005): The heat-shock factor is not activated in mammalian cells exposed to cellular phone frequency microwaves
• Miyakoshi J et al. (2005): Effects of exposure to a 1950 MHz radio frequency field on expression of Hsp70 and Hsp27 in human glioma cells
• Lim HB et al. (2005): Effect of 900 MHz electromagnetic fields on nonthermal induction of heat-shock proteins in human leukocytes
• Czyz J et al. (2004): High frequency electromagnetic fields (GSM signals) affect gene expression levels in tumor suppressor p53-deficient embryonic stem cells
• Pacini S et al. (2002): Exposure to global system for mobile communication (GSM) cellular phone radiofrequency alters gene expression, proliferation, and morphology of human skin fibroblasts
• Leszczynski D et al. (2002): Non-thermal activation of the hsp27/p38MAPK stress pathway by mobile phone radiation in human endothelial cells: Molecular mechanism for cancer- and blood-brain barrier-related effects
• Tian F et al. (2002): Exposure to 2.45 GHz electromagnetic fields induces hsp70 at a high SAR of more than 20 W/kg but not at 5W/kg in human glioma MO54 cells
• Goswami PC et al. (1999): Proto-oncogene mRNA levels and activities of multiple transcription factors in C3H 10T 1/2 murine embryonic fibroblasts exposed to 835.62 and 847.74 MHz cellular phone communication frequency radiation
• Ivaschuk OI et al. (1997): Exposure of nerve growth factor-treated PC12 rat pheochromocytoma cells to a modulated radiofrequency field at 836.55 MHz: effects on c-jun and c-fos expression