この研究は、出芽酵母（Saccharomyces cerevisiae）の細胞増殖に対するマイクロ波照射の影響を調べた。標準化ミリ波導波管に結合したばく露チャンバには、サーモスタットおよび細胞観察のための走査型顕微鏡のステージに取り付けられた。周波数は41.688〜41.707 GHz、8 kHz変調方形波を用い、ばく露時間は10時間連続で行なった。ばく露を受ける細胞は単層培養された。結果として、マイクロ波ばく露は、酵母の細胞増殖速度を、周波数と強度に依存する形で変化させた；細胞増殖は、10〜20％の範囲で増加または減少した、と報告している。
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To investigate the influence of microwave irradiation on the cell growth of the yeast Saccharomyces cerevisiae.
A total of 37 growth experiments were conducted at 1 nW/cm², 27 with and 10 without MW radiation. Further 33 runs were performed at 5 pW/cm².
| 周波数 | 41.6965 GHz |
|---|---|
| タイプ |
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| 偏波 |
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| ばく露時間 | continuous for 10 h |
| Additional information | Several frequencies between 41.688 and 41.707 GHz were applied. |
| Modulation type | cf. additional information |
|---|---|
| Additional information |
modulated at 8 kHz, square wave |
| ばく露の発生源／構造 |
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|---|---|
| チャンバの詳細 | The thermostatted metallic irradiation chamber, coupled by a flexible waveguide with a standardised millimeter wave set-up [Grundler et al., 1983], was mounted on the stage of a scanning microscope. |
| ばく露装置の詳細 | The radiation was guided through a tapered gold-coated quartz needle broadened by quartz carrier plates on both sides and radiated out of a slot (0.4 mm x 1.7 mm) on which the cells were spread in a monolayer. Yeast cells were fixed to the lower surface of a thin transparent agar layer (0.2 mm) filling the inner part of a smaller plastic ring. The agar layer and the cells rested on the quartz needle and the carrier plates. At the opposite surface of the quartz needle, a broader opening for microscopic viewing was covered with a fine metallic mesh suppressing RF leakage. |
| Additional information | The MW field in the radiating slot was monomodal, and the EF was polarized in the y direction, surrounded by the MF in the x-z plane. |
| 周波数 | 41.6965 GHz |
|---|---|
| タイプ |
|
| 偏波 |
|
| ばく露時間 | continuous for 10 h |
| Additional information | Several frequencies between 41.688 and 41.704 GHz were applied. |
| Modulation type | cf. additional information |
|---|---|
| Additional information |
modulated at 8 kHz, square wave |
| ばく露の発生源／構造 |
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|---|
Microwave exposure affects the cell growth rate of the yeast S. cerevisiae in a frequency and intensity dependent manner. Cell growth was either increased or decreased in a range of 10-20%.
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