研究のタイプ: 医学／生物学の研究 (experimental study)

[硝酸塩で培養されたRAW264.7マクロファージで産出された一酸化窒素をUWBパルスは増加させる] med./bio.

Ultra-wideband pulses increase nitric oxide production by RAW 264.7 macrophages incubated in nitrate

著者: Seaman RL, Parker JE, Kiel JL, Mathur SP, Grubbs TR, Prol HK
掲載誌: Bioelectromagnetics 2002; 23 (1): 83-87
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この研究は、超広帯域（UAB）パルスが細胞での一酸化窒素産生に影響するか否かを、UABばく露を受けたRAW 264.7マクロファージ培養液中の亜硝酸塩を測定することで調べた。UABパルス（パルス幅1 ns、パルス繰返し周波数600 Hz）を、SAR推定値0.106 W/ kgで30分間ばく露した。その結果、γインターフェロンおよびリポ多糖類による刺激の有無にかかわらず、UABパルスばく露は、RAW 264.7細胞による一酸化窒素産生を変化させなかった；しかし、RAW 264.7細胞の培地に硝酸塩を添加した場合、一酸化窒素産生はUWBパルスばく露後に増加した；これは、特定の条件下で誘導される一酸化窒素合成酵素へのUWBパルスの作用の可能性を示している、と報告している。

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研究目的（著者による）

To investigate the possible effects of ultra-wideband pulses (UWB) on the cellular nitric oxide (NO) production.

詳細情報

NO is produced by the enzyme nitric oxide synthase (NOS) and was indirectly measured by determination of nitrite concentration in the media. Nitric oxide synthase is induced (iNOS) by γ-interferon and lipopolysaccharides (LPS).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 84.4 MHz–3.08 GHz
Modulation type: pulsed
ばく露時間: continuous for 30 min

ばく露1

主たる特性
周波数 84.4 MHz–3.08 GHz
タイプ
  • electromagnetic field
波形
  • pulsed
特性
  • guided field
ばく露時間 continuous for 30 min
Additional information median frequency 0.51 GHz according to Foster PR. 1995. Antennas and UWB signals. In: Taylor JD, editor. Introduction to ultra-wideband radar systems. Boca Raton: CRC Press. p 147-216.
Modulation
Modulation type pulsed
Pulse width 0.98 ns
Rise time 162 ps
Repetition frequency 600 Hz
Additional information

duration of 0.98 ± 0.03 ns and rise time of 162 ± 9 ps
ばく露装置
ばく露の発生源／構造
  • GTEMセル
チャンバの詳細 Cells were exposed at room temperature (20-25°C) in a custom built GTEM cell, a tapered two conductor transmission line with a square cross section. The UWB exposure system, including the GTEM cell, has been described previously [Lu et al., 1999; Seaman et al., 1999].
ばく露装置の詳細 Pairs of T75 culture flasks with 235 ml PBS were positioned symmetrically on either side of the midline of the GTEM cell. They stood on end on the horizontal ground plane in the region where a pure TEM wave propagated with the flat growth surface facing the source end. Thus, the growth surface was vertical, parallel to the electric field, and orthogonal to the direction of pulse propagation.
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 0.106 W/kg - 推定値 - -
電界強度 103 kV/m peak - - ± 7 kV/m
電界強度 10.6 kV/m peak 推定値 - at the PBS surface
磁束密度 0.33 mT peak 推定値 - -
磁束密度 0.2 nT average over time 推定値 - -

Reference articles

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

An UWB pulse exposure of murine RAW 264.7 macrophage cells, whether stimulated by γ-interferon and lipopolysaccharides or not, did not change the production of nitric oxide. In stimulated cells the NO production increased after UWB exposure when nitrate was added to the media. This indicates a possible action of UWB pulses under certain conditions on iNOS.

研究の種別:

研究助成

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