研究のタイプ: 医学／生物学の研究 (experimental study)

[ヒト肥満細胞株HMC-1におけるプロテインキナーゼCおよび遺伝子発現に対するマイクロ波ばく露後の影響] med./bio.

Effects on protein kinase C and gene expression in a human mast cell line, HMC-1, following microwave exposure

著者: Harvey C, French PW
掲載誌: Cell Biol Int 2000; 23 (11): 739-748
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この研究は、非熱的レベルのマイクロ波ばく露が、ヒト肥満細胞株HMC-1に及ぼす生物学的影響を調べた。細胞へのマイクロ波ばく露には空洞共振器を用い、864.3MHzの連続波を、平均SAR値 7 W / kg、ばく露時間20分間で、3回／日、7日間与えた。ばく露中の細胞培養液の温度は26.5℃に収まっていた。ばく露の影響として、プロテインキナーゼCの局在化への影響；スクリーニングされた588個の遺伝子のうちの3つの遺伝子の発現の変化；影響を受けた遺伝子は、がん原遺伝子c-kit、転写因子ヌクレオシド二リン酸キナーゼBおよびアポトーシス関連遺伝子DAD-1であった；ストレス反応遺伝子では、不安定的な増強が見られた；形態学またはF-アクチン分布への有意な影響は検出されなかった、と報告している。

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研究目的（著者による）

To determine the effect of an electromagnetic field exposure in the microwave range (864.3MHz) on protein kinase C and gene expression in human mast cells.

詳細情報

The cells were allowed to cool during the exposure.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 864.3 MHz
Modulation type: CW
ばく露時間: repeated daily exposure, 3 x 20 min/day at 4-h intervals, for 7 days
  • SAR: 7.3 W/kg average over mass (cf. remarks) (averaged over 20 g of medium)
  • SAR: 12.1 W/kg unspecified (unspecified) (4.9, 11.4, and 12.1 W/kg at three points)

ばく露1

主たる特性
周波数 864.3 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 repeated daily exposure, 3 x 20 min/day at 4-h intervals, for 7 days
Modulation
Modulation type CW
ばく露装置
ばく露の発生源／構造
チャンバの詳細 The exposure chamber [Donnellan et al., 1997] consisted of a cubic aluminium box of 1.1 m internal side length and contained a horizontal loop antenna mounted on a horizontal Masonite shelf located at the mid point of the chamber. The reflective walls formed a resonant chamber, and thus a complex modal field pattern was generated that was taken into account in the experimental design.
ばく露装置の詳細 Cells in 20 ml of culture medium in a 75-cm² flask were placed in the exposure chamber.
Sham exposure A sham exposure was conducted.
Additional information The cells were allowed to cool during the exposure. After 20 min, there was less than 1°C difference between the temperature of the culture medium in the exposed (26.5°C) and sham (25.8°C) flasks, and this difference occurred only during the last 5 min of the exposure.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 7.3 W/kg average over mass 電力損失による決定値 cf. remarks averaged over 20 g of medium
SAR 12.1 W/kg unspecified 測定値 unspecified 4.9, 11.4, and 12.1 W/kg at three points
測定および計算の詳細

The exact frequency was calculated using a frequency counter. Exposimetry characterisation of the exposure chamber was carried out by Telstra, Melbourne [Anderson et al., 1998]. SAR values were determined using two methods: power loss and thermal measurements using millimetre-sized fluoroptic temperature sensors at three locations within the flask filled with 20 g of gelled culture medium to minimise thermal diffusion due to fluid convective currents. Anderson V, Rowley J, Laurence J, 1998. Limited characterisation of an in vitro radiofrequency exposure facility at the Centre for Immunology, St Vincent's Hospital, Sydney. Telstra Research Laboratories, Melbourne, Australia, 29 April 1998.

Reference articles

  • Donnellan M et al. (1997): [835 MHzの電磁放射ばく露による肥満細胞株、RBL-2H3の細胞増殖、形態、分泌能に及ぼす影響]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

There was no significant morphological difference between the control HMC-1 cells and the exposed cells. There was a consistent trend for an increase in the amount of immunoreactive protein kinase C in the membrane fraction of the exposed cells and a concomitant decrease in the cytosolic fraction. Reproducible and significant changes in expression were seen in only three out of a total of 588 genes screened. The results provide evidence that low-level microwave exposure can affect both gene expression and molecules involved in important cell regulatory functions.

研究の種別:

研究助成

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