研究のタイプ: 医学／生物学の研究 (experimental study)

[放射体の近方界および遠方界領域内でのミリ波放射によるマウスの腹膜好中球における活性酸素種生成の抑制] med./bio.

Inhibition of the production of reactive oxygen species in mouse peritoneal neutrophils by millimeter wave radiation in the near and far field zones of the radiator

著者: Gapeyev AB, Safronova VG, Chemeris NK, Fesenko EE
掲載誌: Bioelectrochem Bioenerg 1997; 43 (2): 217-220
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この研究は、ルミノール依存性化学発光法を用いて、マウス腹腔好中球による活性酸素種（ROS）の生成に対する超高周波・低強度電磁界（EMF）の影響を調べた。好中球をオプソニン化されたザイモサンによって活性化した。その結果、EMFは好中球によるROS生成を抑制した；アンテナ近傍界においては、 狭帯域（41.8〜42.05GHz）において、ROS生成の準共振的抑制が観測された； 一方、アンテナの遠方界では、EMF効果の周波数依存性は見られなかった、と報告している。

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研究目的（著者による）

To investigate the effects of electromagnetic irradiation on the production of reactive oxygen species (ROS) in mouse neutrophils in vitro by using luminol-dependent chemiluminescence technique.

詳細情報

The cells were activated by opsonized zymosan.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 41.75–42.15 GHz
Modulation type: CW
ばく露時間: continuous for 40 min
ばく露2: 41.95 GHz
Modulation type: CW
ばく露時間: continuous for 40 min

ばく露1

主たる特性
周波数 41.75–42.15 GHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 40 min
Additional information Eight or nine frequencies were tested for both near and far field conditions.
Modulation
Modulation type CW
ばく露装置
ばく露の発生源／構造
  • 導波管
  • channel radiator with 17.5 x 12.5 mm aperture
チャンバの詳細 Four experimental 200-µl cuvettes were fixed in a holder at room temperature and irradiated from the side of the 0.3-mm thick cuvette bottom to which the cells were spontaneously attached.
ばく露装置の詳細 The distance from the antenna face was 65 mm for near and 400 mm for far field exposure.
Additional information In the near field zone, the holder was positioned at an angle of 75° between the object plane and the wave propagation direction resulting in an SWR between 1 and 1.2 in the whole frequency range used.
パラメータ
測定量 値 種別 Method Mass 備考
電力密度 240 µW/cm² mean 測定値および計算値 - near field
電力密度 150 µW/cm² mean 測定値および計算値 - far field
測定および計算の詳細

In the near field zone, the SAR was determined from the heating rate using a micro thermocouple. The absorbed energy flux density (AEFD) in a thin cell layer of about 0.1-0.2 mm was calculated from the SAR, the real square of the cuvette bottom and the weight of the water solution. The radiation geometry evaluated using IR thermography showed a uniform SAR distribution in the frequency range used. In the far field zone, the AEFD was estimated from the energy flux density measured using a radiation detector. The output wave frequency was controlled using a wavemeter.

ばく露2

主たる特性
周波数 41.95 GHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 40 min
Additional information near and far field
Modulation
Modulation type CW
ばく露装置
ばく露の発生源／構造
  • E1と同じ装置
パラメータ
測定量 値 種別 Method Mass 備考
電力密度 2,400 µW/cm² maximum 測定値および計算値 - near field
電力密度 0.05 µW/cm² minimum 測定値および計算値 - near field
電力密度 150 µW/cm² maximum 測定値および計算値 - far field
電力密度 0.025 µW/cm² minimum 測定値および計算値 - far field
Additional parameter details

Seven energy levels were tested for both near and far field conditions.

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

A frequency-dependent inhibition of ROS production was observed in the near field zone, whereas the inhibition in the far field zone was not frequency-dependent.

研究の種別:

研究助成

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