研究のタイプ: 医学／生物学の研究 (experimental study)

[電磁界によるニワトリ大脳組織からのカルシウムイオン放出におけるイオン要因] med./bio.

Ionic factors in release of 45Ca2+ from chicken cerebral tissue by electromagnetic fields

著者: Bawin SM, Adey WR, Sabbot IM
掲載誌: Proc Natl Acad Sci USA 1978; 75 (12): 6314-6318
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この研究は、脳波の周波数で振幅変調された高周波電磁界を用いて、単離したニワトリ脳組織からの45Ca2 +流出へのばく露の影響を調べた。その結果、ばく露による45Ca2 +流出の増加が観察された；この応答は、実験槽溶液のカルシウム濃度（0〜4.16mM）の変動に敏感ではなかったが、溶液へのH +添加（0.108mM HCl）によって増強された；溶液が正常な重炭酸塩レベル（2.4mM）でない場合、応答は抑制された； 重炭酸塩を含まない溶液にランタンを添加すると電気的応答性は回復したが、45Ca2 +流出増加はなく、代わりに減少した、と報告している。

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研究目的（著者による）

To investigate the effects of 450 MHz AM modulated radiofrequence exposure on the calcium efflux from chicken brain tissue in combination with bathing solutions of different ionic strength.

詳細情報

The aim of the study was to find a better definition of the calcium sites responding to weak electrical stimulation.
First, changes in calcium efflux were studied in relation to calcium concentration in the medium, because calcium-calcium interactions have often been shown to play an important role in the regulation of calcium uptake and release.
Another approach to differentiating possible components of the 45Ca2+ efflux was to attempt modification of calcium release by adding lanthanum. The trivalent lanthanum ion has been found to be effective in blocking calcium flux across excitable membranes.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 450 MHz
Modulation type: AM
ばく露時間: continuous for 20 min

ばく露1

主たる特性
周波数 450 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 20 min
Additional information semi-far field conditions in a low standing-wave field (SWR < 1.2:1)
Modulation
Modulation type AM
Modulation frequency 16 Hz
Modulation depth 90 %
Additional information

sinusoidal
ばく露装置
ばく露の発生源／構造
チャンバの詳細 The horn radiator was lined and terminated with RF-absorbing material to form an anechoic chamber, seven wavelengths long.
パラメータ
測定量 値 種別 Method Mass 備考
電力密度 0.75 mW/cm² - - - -
電界強度 53 V/m - - - in air
電界強度 100 mV/cm - 測定値 - 50-100 mV/cm in the tissue
電力密度 0.375 mW/cm² - - - -
電力密度 2 mW/cm² - - - -
Additional parameter details

A few comparative studies were made with field powers of 0.375 and 2.0 mW/cm².

測定および計算の詳細

Tissue field gradients were measured using an implantable microdiode array.

Reference articles

  • Sheppard AR et al. (1979): [大脳の高分子における長距離秩序のモデル：Sub-ELF およびVHF、UHFの変調電磁界の影響]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

16 Hz AM modulated radiofrequency is capable to stimulate the calcium efflux of pre-incubated isolated chicken brain tissue. Calcium concentration (in the range of 0 - 4.16 mM) in the bathing solution had no significantly effect on calcium efflux, compared with control. Calcium efflux was inhanced by the addition of H+ (HCl (0.108 mM)) and inhibited by the absence of normal bicarbonate levels (2.4 mM HCO3-). The addition of lanthanum (0.5 mM LaCl3) to bicarbonate-free solutions leads to an decreased calcium efflux in irradiated samples.

研究の種別:

研究助成

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