この研究は、ELF電界が孵化した鶏卵にがんを生じ得るかどうかを調べるため、5℃で1-36日間貯蔵した新鮮なニワトリの有精卵を、弱いELF振動電界(1-40V/cm、1-50Hz、2-6日間)の有無で、38℃で5-6日間インキュベートした。別の鶏卵を用いて発達段階を評価した。その結果、予想に反して、生存率が低下する期間における弱いELF電界中でのインキュベートは、胚の生存率および状態を有意に改善した。ELF電界は、それがなければ生存できなかったと思われる一部の胚の生存を維持できた。
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The effects of exposure of chicken eggs to a 1-50 Hz electric field on embryonal development should be investigated. This pilot study was conducted in view of identifying carcinogenic effects of electric fields.
Fertilized, un-incubated White Leghorn eggs were divided into different groups (10-12 eggs each) within two days after laying. Afterwards, eggs from all groups were first stored at 5°C for 1-36 days before being exposed to a 1-50 Hz electric field with 1-40 V/m at 38°C for 1-7 days. A total of 20 groups was tested with different combinations of storage duration at 5°C, exposure duration as well as field frequency and strength. For each of these groups, a separate control group was used, which was incubated at 38°C without exposure to electric field.
ばく露 | パラメータ |
---|---|
ばく露1:
1–50 Hz
ばく露時間:
for 1-7 days
groups 1-9
|
|
ばく露2:
4 Hz
ばく露時間:
for 3 days
group 10
|
|
ばく露3:
2.5 Hz
ばく露時間:
for 5 days
group 11
|
|
ばく露4:
12.5 Hz
ばく露時間:
for 4 days
group 12
|
|
ばく露5:
20 Hz
ばく露時間:
for 5 days
group 13
|
|
ばく露6:
12.5 Hz
ばく露時間:
for 6 days
group 14
|
|
ばく露7:
12.5 Hz
ばく露時間:
for 2 days
group 15
|
|
ばく露8:
12.5 Hz
ばく露時間:
for 3 days
group 16
|
|
ばく露9:
31.25 Hz
ばく露時間:
for 2 days
group 17
|
|
ばく露10:
12.5 Hz
ばく露時間:
for 3 days
group 18
|
|
ばく露11:
12.5 Hz
ばく露時間:
for 2 days
group 19
|
|
ばく露12:
7.7 Hz
ばく露時間:
for 2 days
group 20
|
|
周波数 | 1–50 Hz |
---|---|
タイプ |
|
波形 |
|
ばく露時間 | for 1-7 days |
Additional information | groups 1-9 |
Additional information | storage duration at 5°C prior to exposure: 1-18 days |
ばく露の発生源/構造 |
|
---|---|
チャンバの詳細 | styrofoam-insulated stainless steel box (60 x 45 x 25 cm3) kept at 38°C ± 0.2°C |
ばく露装置の詳細 | box with pre-warmed eggs (n=10-12) insulated by paper trays was placed between two horizontal aluminum plates (25 cm x 30 cm x 1.5 mm) held 10 cm apart by styrofoam spacers; the lower plate was grounded and the upper plate received an oscillating positive charge |
Additional information | eggs from control groups were incubated at 38°C in a similar box 20 cm away from the exposure system |
周波数 | 4 Hz |
---|---|
タイプ |
|
波形 |
|
ばく露時間 | for 3 days |
Additional information | group 10 |
Additional information | storage duration at 5°C prior to exposure: 15 days |
ばく露の発生源/構造 |
|
---|
測定量 | 値 | 種別 | Method | Mass | 備考 |
---|---|---|---|---|---|
電界強度 | 4 V/cm | - | - | - | - |
周波数 | 2.5 Hz |
---|---|
タイプ |
|
波形 |
|
ばく露時間 | for 5 days |
Additional information | group 11 |
Additional information | storage duration at 5°C prior to exposure: 11 days |
ばく露の発生源/構造 |
|
---|
測定量 | 値 | 種別 | Method | Mass | 備考 |
---|---|---|---|---|---|
電界強度 | 1 V/cm | - | - | - | - |
周波数 | 12.5 Hz |
---|---|
タイプ |
|
波形 |
|
ばく露時間 | for 4 days |
Additional information | group 12 |
Additional information | storage duration at 5°C prior to exposure: 14 days |
ばく露の発生源/構造 |
|
---|
測定量 | 値 | 種別 | Method | Mass | 備考 |
---|---|---|---|---|---|
電界強度 | 4 V/cm | - | - | - | - |
周波数 | 20 Hz |
---|---|
タイプ |
|
波形 |
|
ばく露時間 | for 5 days |
Additional information | group 13 |
Additional information | storage duration at 5°C prior to exposure: 20 days |
ばく露の発生源/構造 |
|
---|
測定量 | 値 | 種別 | Method | Mass | 備考 |
---|---|---|---|---|---|
電界強度 | 4 V/cm | - | - | - | - |
周波数 | 12.5 Hz |
---|---|
タイプ |
|
波形 |
|
ばく露時間 | for 6 days |
Additional information | group 14 |
Additional information | storage duration at 5°C prior to exposure: 18 days |
ばく露の発生源/構造 |
|
---|
測定量 | 値 | 種別 | Method | Mass | 備考 |
---|---|---|---|---|---|
電界強度 | 4 V/cm | - | - | - | - |
周波数 | 12.5 Hz |
---|---|
タイプ |
|
波形 |
|
ばく露時間 | for 2 days |
Additional information | group 15 |
Additional information | storage duration at 5°C prior to exposure: 10 days |
ばく露の発生源/構造 |
|
---|
測定量 | 値 | 種別 | Method | Mass | 備考 |
---|---|---|---|---|---|
電界強度 | 4 V/cm | - | - | - | - |
周波数 | 12.5 Hz |
---|---|
タイプ |
|
波形 |
|
ばく露時間 | for 3 days |
Additional information | group 16 |
Additional information | storage duration at 5°C prior to exposure: 12 days |
ばく露の発生源/構造 |
|
---|
測定量 | 値 | 種別 | Method | Mass | 備考 |
---|---|---|---|---|---|
電界強度 | 2 V/cm | - | - | - | - |
周波数 | 31.25 Hz |
---|---|
タイプ |
|
波形 |
|
ばく露時間 | for 2 days |
Additional information | group 17 |
Additional information | storage duration at 5°C prior to exposure: 20 days |
ばく露の発生源/構造 |
|
---|
測定量 | 値 | 種別 | Method | Mass | 備考 |
---|---|---|---|---|---|
電界強度 | 4 V/cm | - | - | - | - |
周波数 | 12.5 Hz |
---|---|
タイプ |
|
波形 |
|
ばく露時間 | for 3 days |
Additional information | group 18 |
Additional information | storage duration at 5°C prior to exposure: 22 days |
ばく露の発生源/構造 |
|
---|
測定量 | 値 | 種別 | Method | Mass | 備考 |
---|---|---|---|---|---|
電界強度 | 4 V/cm | - | - | - | - |
周波数 | 12.5 Hz |
---|---|
タイプ |
|
波形 |
|
ばく露時間 | for 2 days |
Additional information | group 19 |
Additional information | storage duration at 5°C prior to exposure: 25 days |
ばく露の発生源/構造 |
|
---|
測定量 | 値 | 種別 | Method | Mass | 備考 |
---|---|---|---|---|---|
電界強度 | 4 V/cm | - | - | - | - |
周波数 | 7.7 Hz |
---|---|
タイプ |
|
波形 |
|
ばく露時間 | for 2 days |
Additional information | group 20 |
Additional information | storage duration at 5°C prior to exposure: 36 days |
ばく露の発生源/構造 |
|
---|
測定量 | 値 | 種別 | Method | Mass | 備考 |
---|---|---|---|---|---|
電界強度 | 4 V/cm | - | - | - | - |
Increasing storage durations at 5°C without subsequent electric field exposure (control groups) caused a steady loss of embryo viability with a half-life of about 18 days after more than 12 days of storage. Surprisingly, exposure to an electric field after storage increased the embryo viability significantly, resulting in a new half-life of about 21 days after more than 12 days of storage.
However, some of these rescued embryos showed malformations (e.g. two heads) which have not been reported in eggs from this supplier over many years.
The authors conclude that exposure of chicken eggs to a 1-50 Hz electric field might keep embryos alive which would otherwise not have survived. Carcinogenic effects of electric field exposure should be searched in these rescued embryos.
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