この研究は、コムギ(Triticum aestivum L.)のカドミウムストレス耐性にマイクロ波ばく露がどのような影響を与えるかを調べた。種子に0、5、10、および15秒間、マイクロ波(波長125 mm、電力密度126 mW/ cm2、2450 MHz)へのばく露を行った後、実生が7日齢(1枚の葉が完全に開いた状態)になったとき、CdCl2溶液(150 μM)を10日間与えた。多くの生理学的および生化学的特性の変化を測定し、影響を評価した。その結果、150 μMの CdCl2溶液処理により、対照群に比べ、草丈、根の長さ、乾燥重量、AsAおよびGSH濃度、SOD、POD、CAT、およびAPXの活性は低下し、MDA、H2O2の濃度および産生率(算出式は本文参照)は上昇した;しかし、マイクロ波前処理を5または10秒間行った種子では、MDA、H2O2の濃度および産生率の低下、SOD、POD、CAT、APXの活性およびAsA、GSH濃度の上昇が見られ、コムギ実生のカドミウムストレスに対する耐性が与えられたことが示された、と報告している。
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The objectives of the study were 1) to determine the combined effects of microwave pretreatment and cadmium stress on wheat seedlings and 2) to evaluate the effect of seed pretreatment by microwaves on protecting wheat from cadmium (CdCl2) stress damage using plant morphological and physiological indices.
Seeds were exposed to microwaves for 0, 5, 10 and 15 s (each n=1000) and when the seedlings were 7 days old (with one fully expanded leave), they were treated with 150 µM CdCl2 solution for 10 days. On days 2, 4, 6, 8 and 10 leaves and roots were sampled for various analysis.
CdCl2 treatment alone reduced plant height, root length, dry weight, ascorbate and glutathione concentrations and the enzyme activities of peroxidase, superoxide dismutase, catalase, ascorbate peroxidase, and enhanced the concentration of malondialdehyde, hydrogen peroxide and the production of superoxide when compared with the control.
However, seeds with microwave pretreatment for 5 or 10 s conferred a tolerance to cadmium stress in wheat seedlings by decreasing the concentration of malondialdehyde and hydrogen peroxide, the production of superoxide and increasing the enzyme activities of peroxidase, superoxide dismutase, catalase, ascorbate peroxidase and ascorbate and glutathione concentration. Therefore, antioxidative enzymes and antioxidative compounds may be involved in tolerance of wheat seedlings to cadmium stress. The findings also showed that the microwave exposure had a positive physiological effect on the growth and development of cadmium stressed seedlings (significant increase of root length and dry weight).
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