研究のタイプ: 医学／生物学の研究 (experimental study)

[平滑筋肉腫および平滑筋細胞株に対する低強度の定常な無線周波電磁界の影響] med./bio.

Effects of low intensity static electromagnetic radiofrequency fields on leiomyosarcoma and smooth muscle cell lines

著者: Karkabounas S, Havelas K, Kostoula OK, Vezyraki P, Avdikos A, Binolis J, Hatziavazis G, Metsios A, Verginadis I, Evangelou A
掲載誌: Hell J Nucl Med 2006; 9 (3): 167-172
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この研究は、15匹のウィスターラットに作成された3,4-ベンゾピレン誘発性腫瘍から単離された平滑筋肉腫細胞（LSC）を用いて、熱効果を引き起こさない程度の低レベルの高周波電磁界（EMF）がLSC細胞に与える影響を調べた。EMFへの細胞のばく露には、マルチチャネル動的励起装置100 V1（MCDE）と称する装置が使われ、10 kHz〜120 kHzで45分間ばく露を行った。ばく露後の細胞の24時間培養では、細胞増殖の阻害は見られなかったが、48時間培養後には、ばく露群の細胞の増殖は、対照群に比べ、有意に低下した。その時点での生存細胞は、ばく露された全細胞集団の2 %であった。この生存細胞に再びEMFばく露を与えた（45分間のばく露セッションを合計4回）後、フローサイトメトリ検査を行った。上記の一連の実験を5回繰り返した。その結果、このようにEMFの2回ばく露を受けたLSC細胞の45 %にアポトーシスが誘導され、2 %のみが有糸分裂に移行した；しかしこれらの生存細胞は、無ばく露対照細胞と同等の血小板凝集能すなわち転移能を有していた、と報告している。

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研究目的（著者による）

This in vitro study was performed to investigate the athermal effects of a low frequency electromagnetic field on rat leiomyosarcoma cells and smooth muscle cells.

詳細情報

The experiments were carried out with leiomyosarcoma cells which were isolated from tumors (artificially induced via a benzopyrene injection) of 15 Wistar rats. To evaluate the leiomyosarcoma cells potential for metastasis their ability to aggregate platelets was investigated. The smooth muscle cells were isolated from the rats aorta.
The experiments were repeated five times.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 10–120 kHz
ばく露時間: continuous for 45 min

General information

Electromagnetic resonance fingerprints (492 frequencies) of LSC and SMC were recorded before and after their exposure to EMF (10 Hz to 1 MHz).

ばく露1

主たる特性
周波数 10–120 kHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 45 min
Additional information A total of 492 frequencies were used that were derived from a transformation of the electromagnetic resonance fingerprints.
Modulation
Modulation type cf. additional information
Additional information

The electromagnetic resonance fingerprints were modulated using the proper software.
ばく露装置
ばく露の発生源／構造
  • Multi Channel Dynamic Exciter (MCDE)
ばく露装置の詳細 Six Petri dishes with 10 ml of medium were placed into a Faraday apparatus for exposure.
Sham exposure A sham exposure was conducted.
Additional information LSC and SMC cultures were exposed at 72 and 120 h from zero time. After freezing in liquid nitrogen and unfreezing, LSC cultures were again exposed after 24 and 48 h.
パラメータ
測定量 値 種別 Method Mass 備考
電界強度 1.1 V/m - - - 1.1-1.11 ± 0.01 V/m
磁界強度 0.0027 A/m - - - 0.0027-0.0029 ± 0.00005 A/m
Additional parameter details

"The power density of the EMF was 100 mV/cm³ approximately."

測定および計算の詳細

The MCDE contained an electron paramagnetic resonance spectrometer being a scanner-recorder of the electromagnetic resonance frequencies emitted from biological systems exposed to EMF.

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露前
  • ばく露中
  • ばく露後

研究の主なアウトカム（著者による）

The exposure to low frequency electromagnetic field of leiomyosarcoma cells decreased their cell proliferation and induced apoptosis. Investigations of the survived leiomyosarcoma cells after exposure showed resistance to electromagnetic field exposure, indicated by regain of cell proliferation. The leiomysarcoma cells maintained their potential for metastasis.
The smooth muscle cells were not affected by the electromagnetic field exposure.

研究の種別:

研究助成

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