Medical/biological study (experimental study)

Effect of power-frequency magnetic fields on genome-scale gene expression in Saccharomyces cerevisiae med./bio.

By: Nakasono S, Laramee C, Saiki H, McLeod KJ

Published in: Radiat Res 2003; 160 (1): 25-37

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Aim of study (acc. to author)

To clarify the existence of an effect of high-intensity 50 Hz magnetic fields on genome-wide gene expression in yeast.

Background/further details

As positive controls, the cells were exposed to aerobic conditions, heat (40 °C) or minimal medium.

Endpoint

molecular biosynthesis: gene expression

Exposure

- 50/60 Hz
- magnetic field

Exposure	Parameters
Exposure 1: 50 Hz Exposure duration: continuously for 24 h	magnetic flux density: 10 mT effective value (150 mT, 300 mT)

Exposure 1

Main characteristics

Frequency	50 Hz
Type	magnetic field
Waveform	sinusoidal
Exposure duration	continuously for 24 h
Additional info	vertical mf

Exposure setup

Exposure source	coil(s)
Sham exposure	A sham exposure was conducted.

Parameters

Measurand	Value	Type	Method	Mass	Remarks
magnetic flux density	10 mT	effective value	-	-	150 mT, 300 mT

Reference articles

Nakasono S et al. (2000): Effect of ELF magnetic fields on protein synthesis in Escherichia coli K12

Exposed system:

intact cell/cell culture
yeast (Saccharomyces cerevisiae)

Methods Endpoint/measurement parameters/methodology

molecular biosynthesis: gene expression (two-dimensional gel electrophoresis, DNA microarray)

Investigated system:

isolated bio./chem. substance
DNA/RNA
protein

Time of investigation:

after exposure

Main outcome of study (acc. to author)

The magnetic field exposure did not cause significant changes in expression for proteins or genes that were related to heat-shock response, DNA repair, respiration, protein synthesis and the cell cycle.
The data provide no evidence that the magnetic field exposure alters the fundamental mechanism of translation and transcription in eukaryotic cells.

Study character:

medical/biological study
experimental study
full/main study

Study funded by

not stated/no funding

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