To investigate the effects of extremely low frequency electromagnetic fileds (ELF EMF) alone and in combination with thermal stress on the expression of heat shock protein genes in human myeloid leukemia (HL-60) cells in vitro.
Exposure | Parameters |
---|---|
Exposure 1:
50 Hz
Exposure duration:
continuous for 30 min
|
|
Frequency | 50 Hz |
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Type | |
Waveform | |
Exposure duration | continuous for 30 min |
Exposure source |
|
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Chamber | Plastic chamber maintained at 37°C and/or 43°C. |
Setup | The chamber containing the samples was placed in the center of the coils. |
Sham exposure | A sham exposure was conducted. |
Measurand | Value | Type | Method | Mass | Remarks |
---|---|---|---|---|---|
magnetic flux density | 10 µT | minimum | cf. remarks | - | 20 µT, 40 µT, 60 µT, 80 µT, 100 µT and 140 µT +/- 0.4 µT. |
The results of this study show that extremely low frequency electromagnetic fields are capable to induce the expression of genes encoding for heat shock proteins (in particular HSP 70 A, B, and C). This reaction is enhanced by a simultanious thermal stress (43°C for 30 minutes). These effect was found at all different flux densitys tested with a maximum at flux density of 60-80 µT.
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