Medical/biological study (experimental study)

Differentiation of chromaffin cells elicited by ELF MF modifies gene expression pattern med./bio.

By: Olivares-Banuelos T, Navarro L, Gonzalez A, Drucker-Colin R

Published in: Cell Biol Int 2004; 28 (4): 273-279

Aim of study (acc. to author)

This study was performed to identify genes that could be involved in extremely low frequency magnetic field induced chromaffin cell differentiation.

Exposure	Parameters
Exposure 1: 60 Hz Exposure duration: repeated daily exposure, 4 h/day for 7 days	magnetic flux density: 700 µT unspecified

Exposure 1

Main characteristics

Frequency	60 Hz
Type	magnetic field
Waveform	sinusoidal
Exposure duration	repeated daily exposure, 4 h/day for 7 days

Exposure setup

Exposure source	Helmholtz coils
Chamber	Incubator
Setup	Petri dishes were placed between the coils at a distance of 21 cm.
Additional info	The control and NGF group were placed in a separate incubator.

Parameters

Measurand	Value	Type	Method	Mass	Remarks
magnetic flux density	700 µT	unspecified	measured	-	-

Additional parameter details

Background magnetic field was 0.44 µT.

Exposed system:

intact cell/cell culture
chromaffin cells (from neonatal Wistar rats)

Methods Endpoint/measurement parameters/methodology

molecular biosynthesis: gene transcription (RT-PCR, differential display method, Northern blot, sequencing)

Investigated system:

DNA/RNA

Time of investigation:

after exposure

Main outcome of study (acc. to author)

Prevalent down-regulated gene transcription was observed in the exposed and NGF treated cells compared to the control group (53 transcripts with differential expression). The exposure to extremely low frequency magnetic field showed a specific transcription response (among others, 8 up-regulated transcripts), qualitatively different from that elicited by NGF treated cells.
The sequencing of the electromagnetic field differential expressed genes revealed an increased density of cytosine-thymidine-cytosine-thymidine (CTCT) elements in promoters. These elements are thought to be required for electromagnetic field-dependent gene expression.

Study character:

medical/biological study
experimental study
full/main study

Study funded by

Consejo Nacional de Ciencia y Tecnología (CONACyT; National Council for Science and Technology), Mexico
Universidad Nacional Autònoma de México (UNAM)

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