Medical/biological study (experimental study)

The effect of melatonin on the liver of rats exposed to microwave radiation med./bio.

By: Djordjevic B, Sokolovic D, Kocic G, Veljkovic A, Despotovic M, Basic J, Jevtovic-Stoimenov T, Sokolovic DM

Published in: Bratisl Lek Listy 2015; 116 (2): 96-100

Aim of study (acc. to author)

The effects of exposure of rats to a 900 MHz electromagnetic field and co-exposure to melatonin on oxidative stress in the liver should be investigated.

Background/further details

84 rats were divided into 4 groups (n=21 each): 1) control group, 2) administration of melatonin, 3) exposure to microwaves, 4) administration of melatonin and exposure to microwaves. Seven animals of each group were sacrificed and examined after 20, 40 and 60 days of exposure, respectively.
2 mg/kg body weight melatonin was administered intraperitoneally every morning in groups 2 and 4. Animals of the control group received an equal volume of isotonic saline solution.

Endpoint

oxidative stress in the rat liver

Exposure

- 900 MHz
- RF
- mobile communications
- GSM
- co-exposure

Exposure	Parameters
Exposure 1: 900 MHz Modulation type: CW Exposure duration: continuous for 4h/day for 20, 40 or 60 days	magnetic flux density: 4.86 µT minimum (according to abstract 100 µT (?)) magnetic flux density: 8.69 µT maximum (according to abstract 300 µT (?)) electric field strength: 9.88 V/m minimum (according to abstract 54 V/m (?)) electric field strength: 18.356 V/m maximum (according to abstract 160 V/m (?)) SAR: 0.089 W/g mean (whole body)

Exposure 1

Main characteristics

Frequency	900 MHz
Type	electromagnetic field
Charakteristic	near field
Exposure duration	continuous for 4h/day for 20, 40 or 60 days
Additional info	GSM frequency band-mobile transmitting signal

Modulation

Modulation type	CW

Exposure setup

Exposure source	cellular phone
Chamber	plastic cage without metallic fittings
Setup	rats placed in plastic cage containing a mobile phone (Nokia 3110) with antenna positioned downwards; animals were able to move freely during exposure

Parameters

Measurand	Value	Type	Method	Mass	Remarks
magnetic flux density	4.86 µT	minimum	measured	-	according to abstract 100 µT (?)
magnetic flux density	8.69 µT	maximum	measured	-	according to abstract 300 µT (?)
electric field strength	9.88 V/m	minimum	measured	-	according to abstract 54 V/m (?)
electric field strength	18.356 V/m	maximum	measured	-	according to abstract 160 V/m (?)
SAR	0.089 W/g	mean	estimated	whole body	-

Exposed system:

animal
rat/Wistar albino
whole body

Methods Endpoint/measurement parameters/methodology

oxidative stress: lipid peroxidation (reaction of malondialdehyde with thiobarbituric acid), protein oxidation (carbonyl group content), enzyme activity of catalase, xanthine oxidase, alkaline deoxyribonuclease I and acid deoxyribonuclease II (markers for apoptosis); total protein content (Lowry's method) (spectrophotometry)

Investigated system:

isolated bio./chem. substance
liver homogenates

Time of investigation:

during exposure
after exposure

Main outcome of study (acc. to author)

The lipid peroxidation was significantly increased in group 3 (exposure to microwaves) compared to the control group after 40 and 60 days of exposure. At the same time, it was significantly reduced in group 4 (melatonin + microwaves) compared to group 3.
Moreover, protein oxidation was significantly increased after 20, 40 and 60 days, catalase and xanthine oxidase enzyme activity were significantly increased after 40 and 60 days and the enzyme activity of alkaline deoxyribonuclease I was significantly increased after 60 days of exposure in group 3 compared to the control group and group 2 (melatonin only).
The authors conclude that exposure of rats to a 900 MHz electromagnetic field could cause oxidative stress in the liver and that melatonin might have an antioxidant effect by reducing lipid peroxidation.

Study character:

medical/biological study
experimental study
full/main study

Study funded by

Ministry of Education and Science, Serbia

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