Medical/biological study (experimental study)

Modulation of wireless (2.45 GHz)-induced oxidative toxicity in laryngotracheal mucosa of rat by melatonin med./bio.

By: Aynali G, Naziroglu M, Celik O, Dogan M, Yariktas M, Yasan H

Published in: Eur Arch Otorhinolaryngol 2013; 270 (5): 1695-1700

Aim of study (acc. to author)

To investigate the possible protective role of melatonin on oxidative stress generated by exposure to a WiFi-signal in rats.

Background/further details

Four groups (n=8 per group) were examined: 1.) cage control, 2.) sham exposure + saline injection. 3.) exposure and 4.) exposure + melatonin injection.

Endpoint

oxidative stress in mucosa

Exposure

- 2,450 MHz
- 2.45 GHz
- W-LAN/WiFi

Exposure	Parameters
Exposure 1: 2,450 MHz Modulation type: pulsed Exposure duration: continuous for 60 min/d for 30 days (28 days? different indications in the article)	electric field strength: 11.07 V/m effective value (closest point to body) SAR: 0.143 W/kg effective value (whole body) SAR: 4.2 W/kg maximum

Exposure 1

Main characteristics

Frequency	2,450 MHz
Type	electromagnetic field
Waveform	pulsed
Exposure duration	continuous for 60 min/d for 30 days (28 days? different indications in the article)

Modulation

Modulation type	pulsed
Repetition frequency	217 Hz

Exposure setup

Exposure source	half-wave dipole or monopole (varying indications within the text; remark EMF-Portal)
Distance between exposed object and exposure source	1 m
Chamber	exposure system was organized with a special cylindrical strainer which is appropriate for exposure condition and physical size of one rat (length 15 cm, diameter 5 cm), noses of rats were positioned in close contact to the antenna (according to a table in the article: distance between head and antenna 1 m (?)) and the tube was ventilated from head to tail to decrease stress, 8 rats (Figure shows 6 rats (?)) exposed at the same time
Setup	whole exposure system was kept in a Faraday cage
Sham exposure	A sham exposure was conducted.

Parameters

Measurand	Value	Type	Method	Mass	Remarks
electric field strength	11.07 V/m	effective value	measured	-	closest point to body
SAR	0.143 W/kg	effective value	calculated	whole body	-
SAR	4.2 W/kg	maximum	calculated	-	-

Reference articles

Gumral N et al. (2009): Effects of Selenium and L-Carnitine on Oxidative Stress in Blood of Rat Induced by 2.45-GHz Radiation from Wireless Devices

Exposed system:

animal
rat/Wistar
whole body

Methods Endpoint/measurement parameters/methodology

oxidative stress: level of lipid peroxidation (via thiobarbituric acid-reactive substances), level of reduced glutathione, enzyme activity of glutathione peroxidase (spectrophotometry); concentration of total protein (Lowry protein assay, spectroscopy)

Investigated system:

isolated bio./chem. substance
tissue homogenates (mucosa of larynx and trachea)

Time of investigation:

after exposure

Main outcome of study (acc. to author)

In the exposed group, the amount of lipid peroxidation was significantly increased compared to the cage control and the sham exposed group, while an administration of melatonin prevented this effect. No differences in the level of reduced glutathione were found between the groups. The enzyme activity of the glutathione peroxidase was lower (not significantly) in the exposed group than in the cage control and the sham exposure group. However, in the exposure group with melatonin administration, the enzyme activity of the glutathione peroxidase was significantly increased compared to the exposure group without melatonin administration.

Study character:

medical/biological study
experimental study
full/main study
double-blind study

Study funded by

not stated/no funding

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