Medical/biological study (experimental study)

Effect of Extremely Low Frequency Electromagnetic Fields (EMF) on Phospholipase Activity in the Cultured Cells med./bio.

By: Song HS, Kim HR, Ko MS, Jeong JM, Kim YH, Kim MC, Hwang YH, Sohn UD, Gimm YM, Myung SH, Sim SS

Published in: Korean J Physiol Pharmacol 2010; 14 (6): 427-433

Exposure	Parameters
Exposure 1: 60 Hz Exposure duration: continuous for 4 or 16 h	magnetic flux density: 0.1 mT magnetic flux density: 1 mT

Exposure source	coil(s)
Setup	four square coils; cage with three floors arranged vertically; exposure system shielded with ferrite material

Measurand	Value	Type	Method	Mass	Remarks
magnetic flux density	0.1 mT	-	measured	-	-
magnetic flux density	1 mT	-	measured	-	-

Exposed system:

cell function: enzyme activities of phospholipases (phospholipase C (via ³H phosphatidylinositol), phospholipase D (via ³H oleic acid and after treatment with PMA (protein kinase C activator), phospholipase A₂ (via ³H arachidonic acid release, basal and induced by melittin (an endogenous phospholipase A₂ activator), via hydrolysis of ¹⁴C arachidonyl phosphatidylcholine (for cytosolic phospholipase A₂) and via 10-pyrene phosphatidylcholine (for secreted phospholipase A₂; spectrophotometry); scintillation counting).

Investigated system:

Time of investigation:

The basal and melittin-induced arachidonic acid release (phospholipase A₂ enzyme activity) was not affected by the electromagnetic field exposure in both cell lines. There was no change of cytosolic phospholipase A₂ and secreted phospholipase enzyme activity due to exposure. Also both phospholipase C and phospholipase D enzyme activities did not show any change in the two exposed cell lines.
The data indicate that the exposure to electromagnetic fields which is 2.4 fold higher than the limit value of occupational exposure does not induce phospholipase-associated signal pathway in RAW 264.7 cells and RBL 2H3 cells.

Study character:

Patruno A et al. (2011): Kinetic Study on the Effects of Extremely Low Frequency Electromagnetic Field on Catalase, Cytochrome P450 and Inducible Nitric Oxide Synthase in Human HaCaT and THP-1 Cell Lines
Hwang YH et al. (2011): Intracellular Ca Mobilization and Beta-hexosaminidase Release Are Not Influenced by 60 Hz-electromagnetic Fields (EMF) in RBL 2H3 Cells
Patruno A et al. (2010): Extremely low frequency electromagnetic fields modulate expression of inducible nitric oxide synthase, endothelial nitric oxide synthase and cyclooxygenase-2 in the human keratinocyte cell line HaCat: potential therapeutic effects in wound healing
Piacentini MP et al. (2004): Phospholipase C-dependent phosphoinositide breakdown induced by ELF-EMF in Peganum harmala calli
Donato A et al. (2004): Low power microwave interaction with phospholipase C and D signal transduction pathways in myogenic cells
Dibirdik I et al. (1998): Stimulation of Src family protein-tyrosine kinases as a proximal and mandatory step for SYK kinase-dependent phospholipase Cgamma2 activation in lymphoma B cells exposed to low energy electromagnetic fields
Clejan S et al. (1996): Electromagnetic field induced changes in lipid second messengers