EMF-Portal | Study of low-intensity 2450-MHz microwave exposure enhancing the genotoxic effects of mitomycin C using micronucleus test and comet assay in vitro

Study of low-intensity 2450-MHz microwave exposure enhancing the genotoxic effects of mitomycin C using micronucleus test and comet assay in vitro med./bio.

By: Zhang MB, He JL, Jin LF, Lu DQ

Published in: Biomed Environ Sci 2002; 15 (4): 283-290

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Aim of study (acc. to author)

In this study possible synergistic gentoxic interactions of 2450 MHz microwaves and mitomycin C on human lymphocytes were investigated.

Background/further details

Endpoint

Exposure

Exposure	Parameters
Exposure 1: 2.45 GHz Modulation type: CW Exposure duration: 2 h	power density: 50 W/m² unspecified

Exposure 1

Main characteristics

Frequency	2.45 GHz
Type	electromagnetic field
Exposure duration	2 h

Modulation

Modulation type	CW

Exposure setup

Exposure source	Magnetron

Parameters

Measurand	Value	Type	Method	Mass	Remarks
power density	50 W/m²	unspecified	measured	-	-

Reference articles

Garaj-Vrhovac V et al. (1990): The effect of microwave radiation on the cell genome

Exposed system:

intact cell/cell culture

Methods Endpoint/measurement parameters/methodology

Investigated system:

Time of investigation:

after exposure

Main outcome of study (acc. to author)

The results of this study showed that low-intesity microwave exposure alone could neither induce DNA single-strand break nor increase the number of micronucleated cells on human lymphocytes in vitro. Furthermore the experimental results indicated that exposure increases the DNA damage effect induced by mitomycin C, in comet assay in vitro, when exposure preceded mitomycin C exposure. These synergistic effects could only be observed in comet assay, not in micronucleus test.

Study character:

medical/biological study
experimental study
full/main study

Study funded by

International Cooperative Foundation of Science-Technique Bureau of Zhejiang Province, China

National Natural Science Foundation (NSFC), China

Luukkonen J et al. (2010): Combined effects of 872 MHz radiofrequency radiation and ferrous chloride on reactive oxygen species production and DNA damage in human SH-SY5Y neuroblastoma cells

Perrin A et al. (2010): Evaluation of the co-genotoxic effects of 1800 MHz GSM radiofrequency exposure and a chemical mutagen in cultured human cells

Hansteen IL et al. (2009): Cytogenetic effects of exposure to 2.3 GHz radiofrequency radiation on human lymphocytes in vitro

Mazor R et al. (2008): Increased levels of numerical chromosome aberrations after in vitro exposure of human peripheral blood lymphocytes to radiofrequency electromagnetic fields for 72 hours

Baohong W et al. (2007): Evaluating the combinative effects on human lymphocyte DNA damage induced by ultraviolet ray C plus 1.8 GHz microwaves using comet assay in vitro