Male rats were divided in two groups (n=5, respectively): 1) control group and 2) exposure group. Rats were subjected to the behavioral tests after the exposure period (7 days). Afterwards, rats were sacrificed and the brain was taken for biochemical investigations.
Remark EMF-Portal: unclear, if control group was subjected to a sham exposure.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
50 Hz
Exposure duration:
continously for 7 days (24 h/day)
|
| Frequency | 50 Hz |
|---|---|
| Type | |
| Exposure duration | continously for 7 days (24 h/day) |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 10 mT | effective value | measured | - | - |
Both, elevated plus maze and open field test showed an increased level of anxiety in exposed rats. In detail, elevated plus maze showed that motor activity was significantly reduced in exposed rats compared to the control group. Additionally, exposed rats spent more time in closed arms than those of the control group. Similarly, the the open field test showed that exposed rats spent significantly less time in the central area of the open field and significantly more time in the periphery compared to rats from the control group.
In the hypothalamus of exposed rats, the levels of superoxide anion (O2-) and NO2- were significantly increased compared to the control group. No significant difference was found for ONOO−.
The authors conclude that a 50 Hz magnetic field could induce anxiety-like behavior in rats via a mechanism involving O2- and NO2-.
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