60 rats were equally divided into two groups: exposure and sham exposure. After (sham) exposure, both groups were divided into 3 subsets (n=10 each) to minimize the impact from the testing time and the prior testing. One subset of the animals was subjected to the tests in the order of open field test, tail suspension test and Morris water maze, meanwhile, another subset was subjected to the elevated plus maze and forced swim test subsequently. The last subset of both groups was subjected to the fear conditioning test. After these tests, all animals were killed and the brains examined.
polycarbonate ventilated cages (45 cm x 20 cm x 35 cm, L x W x H), changed every 2 weeks
Setup
exposure device (200 cm x 70 cm x 200 cm, L x W x H) was constructed with ABS plastics and utilized bronze wires; a four floors plastic shelf with a size of 140 cm x 70 cm x 200 cm (L x W x H) was placed inside the device; 45-55% relative humidity and 22-25°C room temperature were maintained
general health (daily inspection), infections (scan for certain pathogens; every 3 months), food and water intake (every 2 weeks), body weight (every 4 weeks), brain weight
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