Medical/biological study (experimental study)

Influence of an Extremely Low Frequency Magnetic Field (ELF-EMF) on Antioxidative Vitamin E Properties in AT478 Murine Squamous Cell Carcinoma Culture In Vitro med./bio.

By: Polaniak R, Buldak RJ, Karon M, Birkner K, Kukla M, Zwirska-Korczala K, Birkner E

Published in: Int J Toxicol 2010; 29 (2): 221-230

Exposure	Parameters
Exposure 1: 3 Hz–3 kHz Exposure duration: continuous for 16 min	magnetic flux density: 0.11 mT

Exposure 1

Main characteristics

Frequency	3 Hz–3 kHz
Type	magnetic field
Exposure duration	continuous for 16 min

Exposure setup

Exposure source	MRS 2000 device

Parameters

Measurand	Value	Type	Method	Mass	Remarks
magnetic flux density	0.11 mT	-	measured	-	-

Exposed system:

intact cell/cell culture
AT478 (murine squamous cell carcinoma cells)

Methods Endpoint/measurement parameters/methodology

cell function: antioxidant enzyme activity (manganese- and copper-zinc-containing superoxide dismutase and glutathione peroxidase) and lipid peroxidation (level of malondialdehyde)

Investigated system:

cell supernatants

Time of investigation:

after exposure

Main outcome of study (acc. to author)

Following vitamin E treatment, antioxidant enzyme activity was increased compared with the untreated control group. Extremely low frequency electromagnetic field exposure alone or in combination with vitamin E increased both superoxide dismutase enzyme activities and glutathione peroxidase enzyme activity in comparison to the control group.
The data suggest that extremely low frequency electromagnetic fields alter antioxidative enzyme activities in AT478 tumor cells. This study confirms the role of vitamin E in decreasing susceptibility to lipid peroxidation (decreased levels of malondialdehyde) in AT478 tumor cells.
Further studies should be conducted to determine the influence of extremely low frequency electromagnetic field exposure and vitamin E on cell proliferation processes and chemosensitivity of the cancer cells to commonly used cytostatics causing reactive oxygen species-mediated damage (i.e. high levels of glutathione peroxidase in cancer cells lead to cancer cell survival via effective removal of hydrogen peroxide).

Study character:

medical/biological study
experimental study
full/main study

Study funded by

not stated/no funding

Hong MN et al. (2012): Extremely Low Frequency Magnetic Fields Do Not Elicit Oxidative Stress in MCF10A Cells
Buldak RJ et al. (2012): Short-term exposure to 50 Hz ELF-EMF alters the cisplatin-induced oxidative response in AT478 murine squamous cell carcinoma cells
Eleuteri AM et al. (2009): 50 Hz extremely low frequency electromagnetic fields enhance protein carbonyl groups content in cancer cells: effects on proteasomal systems
Di Loreto S et al. (2009): Fifty hertz extremely low-frequency magnetic field exposure elicits redox and trophic response in rat-cortical neurons
Falone S et al. (2008): Chronic exposure to 50Hz magnetic fields causes a significant weakening of antioxidant defence systems in aged rat brain
Güler G et al. (2008): The protective effects of N-acetyl-L-cysteine and Epigallocatechin-3-gallate on electric field-induced hepatic oxidative stress
Turkozer Z et al. (2008): Effects of exposure to 50 Hz electric field at different strengths on oxidative stress and antioxidant enzyme activities in the brain tissue of guinea pigs
Sahebjamei H et al. (2007): Effects of magnetic field on the antioxidant enzyme activities of suspension-cultured tobacco cells
Falone S et al. (2007): Fifty hertz extremely low-frequency electromagnetic field causes changes in redox and differentiative status in neuroblastoma cells
Seyhan N et al. (2006): In vivo effects of ELF MFs on collagen synthesis, free radical processes, natural antioxidant system, respiratory burst system, immune system activities, and electrolytes in the skin, plasma, spleen, lung, kidney, and brain tissues
Zwirska-Korczala K et al. (2005): Effect of extremely low frequency of electromagnetic fields on cell proliferation, antioxidative enzyme activities and lipid peroxidation in 3T3-L1 preadipocytes--an in vitro study
Zwirska-Korczala K et al. (2004): Influence of extremely-low-frequency magnetic field on antioxidative melatonin properties in AT478 murine squamous cell carcinoma culture

Influence of an Extremely Low Frequency Magnetic Field (ELF-EMF) on Antioxidative Vitamin E Properties in AT478 Murine Squamous Cell Carcinoma Culture In Vitro med./bio.

Aim of study (acc. to author)

Endpoint

Exposure