Medical/biological study (experimental study)

Effect of extremely low frequency of electromagnetic fields on cell proliferation, antioxidative enzyme activities and lipid peroxidation in 3T3-L1 preadipocytes--an in vitro study med./bio.

By: Zwirska-Korczala K, Jochem J, Adamczyk-Sowa M, Sowa P, Polaniak R, Birkner E, Latocha M, Pilc K, Suchanek R

Published in: J Physiol Pharmacol 2005; 56: 101-108

Exposure	Parameters
Exposure 1: 180–195 Hz Exposure duration: continuous for 36 min	magnetic flux density: 120 µT unspecified

Exposure 1

Main characteristics

Frequency	180–195 Hz
Type	magnetic field
Waveform	sawtooth-shaped
Exposure duration	continuous for 36 min

Exposure setup

Exposure source	magnetostimulation device (VIOFOR JPS)

Parameters

Measurand	Value	Type	Method	Mass	Remarks
magnetic flux density	120 µT	unspecified	unspecified	-	-

Exposed system:

intact cell/cell culture
3T3-L1 preadipocyte cells

Methods Endpoint/measurement parameters/methodology

molecular biosynthesis: malondialdehyde concentration (colorimetric assay)
cell function: antioxidative enzyme activities (manganese- and copper-zinc-containing superoxide dismutase; glutathione peroxidase and glutathione reductase; catalase)
cell viability/cell division/proliferation: cell proliferation; cell viability (trypan blue stain)

Investigated system:

isolated bio./chem. substance
intact cell/cell culture

Time of investigation:

after exposure

Main outcome of study (acc. to author)

Extremely low frequency magnetic field exposure lasting 36 minutes daily failed to influence cell count after 24 h and 48 h of incubation.
After 24 h, in the magnetic field exposed group, manganese- and copper-zinc-containing superoxide dismutase enzyme activities were decreased, catalase activity was increased, whereas there were no significant differences in glutathione peroxidase and glutathione reductase activities in comparison to the control. After 48 h of incubation, all enzyme activities were reduced, except for glutathione reductase, in which no changes were found.
Malondialdehyde concentration at 24 h after incubation with the magnetic field exposure was significantly higher in comparison to the control. After 48 h of incubation, malondialdehyde levels were significantly lower in both groups (with no differences between the groups without and with magnetic field exposure).
The authors conclude that extremely low frequency magnetic field influences antioxidative enzyme activities and increases lipid peroxidation in these preadipocyte cultures.

Study character:

medical/biological study
experimental study
full/main study

Study funded by

not stated/no funding

Calcabrini C et al. (2017): Effect of extremely low-frequency electromagnetic fields on antioxidant activity in the human keratinocyte cell line NCTC 2544
Mahmoudinasab H et al. (2016): Effects of extremely low-frequency electromagnetic field on expression levels of some antioxidant genes in human MCF-7 cells
Reale M et al. (2014): Neuronal cellular responses to extremely low frequency electromagnetic field exposure: implications regarding oxidative stress and neurodegeneration
Ma S et al. (2013): Protective Effects of Low-Frequency Magnetic Fields on Cardiomyocytes from Ischemia Reperfusion Injury via ROS and NO/ONOO(-)
Hong MN et al. (2012): Extremely Low Frequency Magnetic Fields Do Not Elicit Oxidative Stress in MCF10A Cells
Patruno A et al. (2012): Activity of matrix metallo proteinases (MMPs) and the tissue inhibitor of MMP (TIMP)-1 in electromagnetic field-exposed THP-1 cells
Buldak RJ et al. (2012): Short-term exposure to 50 Hz ELF-EMF alters the cisplatin-induced oxidative response in AT478 murine squamous cell carcinoma cells
Patruno A et al. (2011): Kinetic Study on the Effects of Extremely Low Frequency Electromagnetic Field on Catalase, Cytochrome P450 and Inducible Nitric Oxide Synthase in Human HaCaT and THP-1 Cell Lines
Yang Y et al. (2010): EMF acts on rat bone marrow mesenchymal stem cells to promote differentiation to osteoblasts and to inhibit differentiation to adipocytes
Polaniak R et al. (2010): Influence of an Extremely Low Frequency Magnetic Field (ELF-EMF) on Antioxidative Vitamin E Properties in AT478 Murine Squamous Cell Carcinoma Culture In Vitro
Farina M et al. (2010): ELF-EMFs induced effects on cell lines: controlling ELF generation in laboratory
Eleuteri AM et al. (2009): 50 Hz extremely low frequency electromagnetic fields enhance protein carbonyl groups content in cancer cells: effects on proteasomal systems
Falone S et al. (2008): Chronic exposure to 50Hz magnetic fields causes a significant weakening of antioxidant defence systems in aged rat brain
Falone S et al. (2007): Fifty hertz extremely low-frequency electromagnetic field causes changes in redox and differentiative status in neuroblastoma cells
Seyhan N et al. (2006): In vivo effects of ELF MFs on collagen synthesis, free radical processes, natural antioxidant system, respiratory burst system, immune system activities, and electrolytes in the skin, plasma, spleen, lung, kidney, and brain tissues
Zwirska-Korczala K et al. (2004): Influence of extremely-low-frequency magnetic field on antioxidative melatonin properties in AT478 murine squamous cell carcinoma culture

Effect of extremely low frequency of electromagnetic fields on cell proliferation, antioxidative enzyme activities and lipid peroxidation in 3T3-L1 preadipocytes--an in vitro study med./bio.

Aim of study (acc. to author)

Endpoint

Exposure