DNA fragmentation in human fibroblasts under extremely low frequency electromagnetic field exposure med./bio.

Aim of study (acc. to author)

Background/further details

Endpoint

• genotoxicity/mutation: DNA damage, DNA strand breaks

Exposure

• • 50/60 Hz
  • magnetic field
  • low frequency
• • power transmission line

Extended view Compact view

Methods Endpoint/measurement parameters/methodology

• genotoxicity/mutation: DNA damage, DNA strand breaks (alkaline Comet assay and Fpg (formamidopyrimidine-DNA-glycosylase)-modified Comet assay (to measure oxidative stress-induced DNA damage)
• molecular biosynthesis: DNA synthesis (bromodeoxyuridine incorporation; flow cytometry)
• cell viability/cell division/proliferation: cell cycle analysis (propidium iodide stain; flow cytometry); apoptosis (Annexin V-FITC or propidium iodide staining; flow cytometry)

Main outcome of study (acc. to author)

Study funded by

• Forschungsstiftung Mobilkommunikation (FSM; Research Foundation on Mobile Communication; at the Swiss Federal Institute of Technology Zurich (ETH)), Switzerland
• Nationales Forschungsprogramm NFP 57 (National Research Programme NRP 57), Switzerland
• Swiss National Science Foundation (SNF)

Replicated studies

• Ivancsits S et al. (2003): Intermittent extremely low frequency electromagnetic fields cause DNA damage in a dose-dependent way
• Ivancsits S et al. (2002): Induction of DNA strand breaks by intermittent exposure to extremely-low-frequency electromagnetic fields in human diploid fibroblasts

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