1) To study wether 50 Hz electric fields (12 kV/m) induce nitrosative stress and oxidative stress in guinea pigs lung tissue
2) To study the possible effects of extremely low frequency electric fields on collagen synthesis.
Additionally, the effects of antioxidant treatment such as epigallocatechin-3-gallate and N-acetyl-L-cysteine were investigated.
60 guinea pigs were divided into six groups: 1) control group (with saline injection), 2) epigallocatechin-3-gallate injection alone, 3) N-acetyl-L-cysteine injection alone, 4) saline injection before exposure, 5) epigallocatechin-3-gallate injection before exposure, and 6) N-acetyl-L-cysteine before exposure.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
50 Hz
Exposure duration:
8 h/day for 7 days
|
|
animals were divided into 6 groups: i) control ii) injection of 25 mg/kg EGCG iii) injection of 300 mg/kg NAC iv) EMF exposure v) injection of EGCG + EMF exposure vi) injection of NAG + EMF exposure
| Frequency | 50 Hz |
|---|---|
| Type | |
| Exposure duration | 8 h/day for 7 days |
| Exposure source |
|
|---|---|
| Setup | copper plates mounted on the top and bottom of the wooden cage |
| Sham exposure | A sham exposure was conducted. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| electric field strength | 12 kV/m | - | measured and calculated | - | - |
While protein carbonyl content levels significantly increased, insignificant changes were observed in heme oxygenase-1, malondialdehyde, and nitric oxide levels for electric field exposure groups compared to the control group. The data showed no significant changes in these parameters on the electric field group compared to the group where epigallocatechin-3-gallate and N-acetyl-L-cysteine were separately applied along with electric field.
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