The purpose of this in vivo study was to investigate the possible effects of extremely low-frequency magnetic fields on nitric oxide (NO) level in rats.
Twenty-seven male, four months old rats were used. Animals were exposed to three different magnetic flux densities, on the basis of the magnetic fields exposure limits of ICNIRP (100 μT: general public limit, 500 μT: occupational limit).
| Exposure | Parameters |
|---|---|
|
Exposure 1:
50 Hz
Exposure duration:
2 h/day for 10 months
|
|
|
Exposure 2:
50 Hz
Exposure duration:
2 h/day for 10 months
|
|
| Frequency | 50 Hz |
|---|---|
| Type | |
| Exposure duration | 2 h/day for 10 months |
| Exposure source | |
|---|---|
| Setup | pair of Helmholtz coils with a diameter of 25 cm with 225 turns of 1 mm insulated copper wire, 25 cm apart, in a 130 cm x 65 cm x 80 cm Faraday cage; rats in 17 cm x 17 cm x 25 cm methacrylate boxes between the coils |
| Sham exposure | A sham exposure was conducted. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 100 µT | - | measured | - | - |
| Frequency | 50 Hz |
|---|---|
| Type | |
| Exposure duration | 2 h/day for 10 months |
| Exposure source |
|
|---|---|
| Sham exposure | A sham exposure was conducted. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 500 µT | - | measured | - | - |
A significant reduction was observed in nitrite levels among the extremely low frequency exposure groups (100 μT and 500 μT) of rats compared to sham exposed rats after exposure for 10 months.
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