To evaluate the effects of extremely low frequency magnetic field on beta-cell survival and function, a insulin-secreting cell line, which exhibits responsiveness to glucose was cultured under sham exposure and extremely low frequency magnetic field conditions, and cell survival and function was assessed.
In diabetes, the loss of mass and function of beta cells, which are insulin-secreting components of pancreatic islets of Langerhans, is crucial.
Cells were cultured with 40 mg/dl and 100 mg/dl glucose.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
60 Hz
Exposure duration:
continuous for 2 days or 5 days
|
|
| Frequency | 60 Hz |
|---|---|
| Type | |
| Waveform | |
| Exposure duration | continuous for 2 days or 5 days |
| Exposure source | |
|---|---|
| Setup | Helmholtz coil inside CO2 incubator, cylindrical exposure space with 20 cm diameter and a height of 14 cm, field vertical |
| Sham exposure | A sham exposure was conducted. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 5 mT | - | measured | - | - |
Exposure to extremely low frequency magnetic field at 5 mT affected beta-cell survival and function; exposure at 5 mT for 5 days in the absence of glucose increased cell number, exposures for 2 days in the absence of glucose and for 5 days with 100 mg/dl glucose increased insulin secretion in the culture medium, and exposure for 2 and 5 days with 40 and 100 mg/dl glucose increased intracellular insulin concentration in HIT-T15 cells.
The increase in cell number under apoptotic culture conditions (absence of glucose) by exposure to extremely low frequency magnetic field could lead to new therapeutic concepts in the treatment of diabetes.
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