Medical/biological study (experimental study)

Investigation of protein expression in magnetic field-treated human glioma cells med./bio.

By: Kanitz MH, Witzmann FA, Lotz WG, Conover D, Savage Jr RE

Published in: Bioelectromagnetics 2007; 28 (7): 546-552

Aim of study (acc. to author)

This study was performed to investigate possible effects of low frequency magnetic field on protein expression in human glial cells in vitro.

Background/further details

The cells were exposed to magnetic field plus/without epidermal growth factor (EGF, 10 ng/ml). The experiments were replicated twice.

Endpoint

cell function: protein expression

Exposure

- 50/60 Hz
- magnetic field
- therapeutic/medical device
- electrotherapy

Exposure	Parameters
Exposure 1: 60 Hz Exposure duration: 3 h continuous	magnetic flux density: 1.2 µT

General information

cells were treated in four groups: i) sham ii) exposure to 1.2 µT iii) sham + EGF (10 mg/ml) iv) exposure to 1.2 µT + EGF

Exposure 1

Main characteristics

Frequency	60 Hz
Type	magnetic field
Exposure duration	3 h continuous

Exposure setup

Exposure source	Helmholtz coils
Setup	two concentric Helmholtz coils systems with the inner coil for exposure and the outer coil to limit stray fields
Sham exposure	A sham exposure was conducted.

Parameters

Measurand	Value	Type	Method	Mass	Remarks
magnetic flux density	1.2 µT	-	measured	-	-

Exposed system:

intact cell/cell culture
SF767 (human glioma cell line)

Methods Endpoint/measurement parameters/methodology

cell function: protein expression (gel electrophoresis, Coomassie blue stain), protein identification (peptide mass fingerprinting)

Investigated system:

isolated bio./chem. substance
protein

Time of investigation:

after exposure

Main outcome of study (acc. to author)

The mean abundance of 10 identified proteins was altered following magnetic field exposure (three proteins increased, seven proteins decreased).
The co-exposure to epidermal growth factor increased the expression of two proteins and decreased the expression of four proteins.
The results indicate that the analysis of differentially expressed proteins in the human glial cells (SF767) may serve as markers for biological changes caused by the exposure to magnetic fields.

Study character:

medical/biological study
experimental study
full/main study
blind study

Study funded by

not stated/no funding

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