To study the functional interaction of a 50 Hz, 0.4 mT magnetic field with a membrane receptor, epidermal growth factor receptor, mainly at the molecular level.
Epidermal growth factor receptor (EGFR) is a transmembrane glycoprotein expressed in many types of mammalian cell surfaces. An EGFR monomer contains an intracellular domain with tyrosine kinase which can be specifically inhibited by an EGFR tyrosine kinase-specific inhibitor PD153035 to block EGFR activation and to prevent intracellular signal pathway.
Purified EGFR (5 mg/ml) or cells were divided into three groups: 1) sham-exposed group, 2) magnetic field-exposed group, or 3) the PD inhibitor pretreated group with different concentrations.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
50 Hz
Exposure duration:
continuous for 30 min
|
|
| Frequency | 50 Hz |
|---|---|
| Type | |
| Waveform | |
| Exposure duration | continuous for 30 min |
| Exposure source |
|
|---|---|
| Setup | The magnetic field was fairly uniform (± 0.012 mT) over the exposure area between the plates. The whole set-up was placed in a CO2 incubator maintained at 37°C and was shield from external field. |
| Sham exposure | A sham exposure was conducted. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 0.4 mT | unspecified | measured | - | - |
The data showed that the magnetic field exposure induced purified EGF receptors to form clusters (i.e. at least two monomers form a cluster), free from cell membrane and EGF binding. The EGFR tyrosine kinase inhibitor evidently blocks the ligand-free-clustering effect.
The findings indicate that the tyrosine kinase domain of EGFR is a likely candidate site for the magnetic field interaction.
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