Study type: Medical/biological study (experimental study)

Electromagnetic field of extremely low frequency decreased adenylate kinase activity in retinal rod outer segment membranes med./bio.

By: Ravera S, Repaci E, Morelli A, Pepe IM, Botter R, Beruto D
Published in: Bioelectrochemistry 2004; 63 (1-2): 317-320
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Aim of study (acc. to author)

To investigate the effects of an extremely low frequency electromagnetic field on adenylate kinase activity of bovine retina in vitro.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 75 Hz
Modulation type: pulsed
Exposure duration: 2 min
Exposure 2: 75 Hz
Modulation type: pulsed
Exposure duration: 5 min
Exposure 3: 75 Hz
Modulation type: pulsed
Exposure duration: 10 min

Exposure 1

Main characteristics
Frequency 75 Hz
Type
Waveform
Exposure duration 2 min
Modulation
Modulation type pulsed
Pulse width 13.3 ms
Duty cycle 10 %
Pulse type rectangular
Exposure setup
Exposure source
Setup pair of Helmholtz coils, kept 12 cm apart; samples placed between the coils
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 250 µT unspecified measured - -

Exposure 2

Main characteristics
Frequency 75 Hz
Type
Waveform
Exposure duration 5 min
Modulation
Modulation type pulsed
Pulse width 13.3 ms
Duty cycle 10 %
Pulse type rectangular
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 250 µT unspecified measured - -

Exposure 3

Main characteristics
Frequency 75 Hz
Type
Waveform
Exposure duration 10 min
Modulation
Modulation type pulsed
Pulse width 13.3 ms
Duty cycle 10 %
Pulse type rectangular
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 250 µT unspecified measured - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The rod outer segment of bovine retina contains two forms of adenylate kinase activity, one bound to membranes and the other one soluble in the cytosol. Extremely low frequency electromagnetic field exposure leads to a 55% decrease of the former one. If membrane fractions were artifically solubilised by the use of the detergent triton, the enzyme activity was just slightly affected. Also negligible effects were observed for the cytosolic isoform.
These findings suggest the importance of the cell membrane for the interaction of an extremely low frequency electromagnetic field exposure with enzyme inactivation.

Study character:

Study funded by

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