To determine if in vitro exposure to hyperthermia or to non-thermal radiofrequency irradiation induces changes in the heat shock protein 27 (HSP27) phosphorylation of human cell lines.
Five different isoforms of the heat shock protein 27 (HSP27) were considered.
Each experiment was repeated three times.
| Frequency | 847 MHz |
|---|---|
| Type | |
| Charakteristic |
|
| Exposure duration | continuous for 1, 2 or 24 h |
| Exposure source |
|
|---|---|
| Distance between exposed object and exposure source | 292 mm |
| Chamber | A detailed description of the radial transmission line used for exposure has been reported in several publications [Moros et al., 1998 and 1999]. The RTL was formed by two horizontal parallel conductive plates, 43 mm apart. The 6 mm thick bottom AL plate was highly thermally conductive thus minimising temperature gradients among flasks. A central conical AL antenna emitted TEM waves propagating radially outward and terminated by an annulus of RF-absorbing foam material braced by a thin lamina of perforated aluminium at 533 mm from the centre. |
| Setup | A total of 16 T-75 culture flasks could be distributed angularly around the antenna with their centres located at a radius of 292 mm. The cell layer was between 247 and 338 mm from the centre. Each flask was dielectrically loaded with a 3 mm thick shim of alumina (Al2O3) in order to boost and homogenise the SAR distribution. |
| Sham exposure | A sham exposure was conducted. |
| Additional info | Cells in T-75 tissue culture flasks containing 40 ml of medium (height 5 mm) were exposed to RF radiation at 37°C or heat shock (2 h at 41°C or 30 min at 45°C). All heat shock and RF exposure conditions were analysed simultaneously along with a matched incubator control sample. |
| Frequency | 900 MHz |
|---|---|
| Type | |
| Charakteristic |
|
| Exposure duration | continuous for 1, 2 or 5 h |
| Exposure source |
|
|---|---|
| Sham exposure | A sham exposure was conducted. |
The exposure to hyperthermia (30 minutes at 45 °C acute, two hours at 45 °C chronic) significantly increased heat shock protein 27 (HSP27) phosphorylation.
No significant differences in the levels of heat shock protein 27 (HSP27) phosphorylation were observed in the exposure of HeLa S3 cells to TDMA.
The exposure of EA.hy926 cells to 900 MHz GSM did not induce significant differences in the levels of heat shock protein 27 (HSP27) phosphorylation.
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