To study the effects of pulsed electromagnetic fields (PEMF) on cell proliferation, polyamine trend, and c-myc and c-fos gene expression of human osteoblast-like cell cultures.
Pulsed electromagnetic field stimulation promotes the healing of fractures in humans. The processes of tissue repair include protein synthesis and cell differentiation. The polyamines are compounds playing a relevant role in both protein synthesis processes and cell differentiation through c-myc and c-fos gene activation.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
75 Hz
Modulation type:
pulsed
Exposure duration:
continuous for 0.5 h, 1 h, 3 h, 6 h, 12 h and 24 h
|
|
| Frequency | 75 Hz |
|---|---|
| Type | |
| Waveform | |
| Exposure duration | continuous for 0.5 h, 1 h, 3 h, 6 h, 12 h and 24 h |
| Modulation type | pulsed |
|---|---|
| Pulse width | 1.3 ms |
| Duty cycle | 10 % |
| Exposure source | |
|---|---|
| Chamber | 5% CO2 Incubator maintained at 37°C |
| Setup | plates with cells placed between the coils |
| Sham exposure | A sham exposure was conducted. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| electric field strength | 2 mV/m | peak value | calculated | - | - |
| magnetic flux density | 2.3 mT | peak value | measured | - | - |
The pulsed electromagnetic field (PEMF) increased [³H]-thymidine incorporation, while putrescine decreased after treatment; spermine and spermidine were not significantly affected. c-myc was activated after 1 h and downregulated thereafter, while c-fos mRNA levels increased after 0.5 h and then decreased.
Putrescine, spermidine, spermine trends, and [³H]-thymidine incorporation were significantly related to pulsed electromagnetic field (PEMF) treatment.
These observations provide evidence that in vitro pulsed electromagnetic fields affects the mechanisms involved in cell proliferation and cell differentiation.
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