To examine the effect of an exposure to extremely low frequency magnetic fields on the development of blood vessels of the yolk sac membrane in quail embryos.
The eggs were incubated (37.5°C) for 96 hours. On the second day of incubation, a small window was cut into the egg shell and covered with a special foil, hence the vascular network could be photographed. Exposure started after an incubation period of 48 hours. Observations were performed after 72 and 96 hours.
6 groups of eggs were examined: 1.) control group (n=7), 2.) 3 x 1 h exposure daily with a 7 h interval between exposures (n=6), 3.) 3 x 2 h exposure daily with a 6 h interval between exposures (n=7), 4.) 3 x 3 h exposure daily with a 5 h interval between exposures (n=14), 5.) 3 x 4 h daily with a 4 h interval between exposures (n=10) and 6.) continuous for 24 h daily (n=15).
| Exposure | Parameters |
|---|---|
|
Exposure 1:
60 Hz
Exposure duration:
intermittent for 3, 6, 9, 12 or 24 h/day for 2 days (see add. information)
|
|
| Frequency | 60 Hz |
|---|---|
| Type | |
| Waveform | |
| Exposure duration | intermittent for 3, 6, 9, 12 or 24 h/day for 2 days (see add. information) |
| Exposure source | |
|---|---|
| Setup | pair of Helmholtz coils with a diameter of 38 cm and 154 turns of wire each; connected in parallel; eggs placed in an incubator along the horizontal axis between the coils |
| Additional info | exposure time: group I: 3 x 1 h/day with a 7 h interval between exposures group II: 3 x 2 h/day with a 6 h interval between exposures group III: 3 x 3 h/day with a 5 h interval between exposures group IV: 3 x 4 h/day with a 4 h interval between exposures group V: continuous for 24 h/day |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 1.13 mT | effective value | measured | - | in the coil's center |
| magnetic flux density | 0.9 mT | effective value | measured | - | at the coil's edge |
In the groups exposed for 3 x 2 h/day and 3 x 3 h/day (groups 3 and 4), the development of the vascular network was inhibited after 72 hours of incubation compared to the control group. No differences between the groups were detected after an incubation time of 96 hours.
The authors conclude that exposure to an extremely low frequency magnetic field could influence the development of blood vessels in a window effect.
This website uses cookies to provide you the best browsing experience. By continuing to use this website you accept our use of cookies.
