The effects of exposure of Japanese quail embryos to a 60 Hz magnetic field on the development of the embryonic body and vascular network of the yolk sac should be investigated.
A total of 30 eggs of Japanese quail were divided into the following groups (n=10, respectively): 1) exposure to a 0.16 mT magnetic field, 2) exposure to a 0.65 mT magnetic field, 3) control group. After 24 h of egg incubation, an opening of approximately 3 cm2 was made with sterile scissors and it was covered with parafilm. This opening allowed the embryo and yolk sac membrane to be visualized. Exposure to the magnetic field startet at 54 h of incubation and finished exactly at 72 h.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
60 Hz
Exposure duration:
3 x 2 hours, with 6-hour intervals in between resulting in a total exposure of 6 h
|
|
|
Exposure 2:
60 Hz
Exposure duration:
3 x 2 hours, with 6-hour intervals in between resulting in a total exposure of 6 h
|
|
| Frequency | 60 Hz |
|---|---|
| Type | |
| Exposure duration | 3 x 2 hours, with 6-hour intervals in between resulting in a total exposure of 6 h |
| Exposure source | |
|---|---|
| Setup | eggs were placed in an incubator (temperature of 37.5°C and humidity above 60%) associated with a pair of Helmholtz coils |
| Sham exposure | A sham exposure was conducted. |
| Additional info | eggs of the control group were incubated in the same incubator associated with the Helmholtz coils turned off |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 0.16 mT | - | measured | - | - |
| Frequency | 60 Hz |
|---|---|
| Type | |
| Exposure duration | 3 x 2 hours, with 6-hour intervals in between resulting in a total exposure of 6 h |
| Exposure source |
|
|---|
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| magnetic flux density | 0.65 mT | - | measured | - | - |
Embryos exposed to the 0.65 mT magnetic field (group 2) showed a significantly smaller body area and percentage cephalic length compared to the control group.
The authors concluded that exposure of Japanese quail embryos to a 60 Hz magnetic field showed no effects on vascular network of the yolk sac but might decrease the development of the embryonic body.
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