To study the effects of acute exposure to electromagnetic fields in the microwave range on primary rat neocortical astroglial cell cultures.
Primary cultures of astrocytes were prepared from the neocortex of newborn rat brains (Wistar rats).
Exposure | Parameters |
---|---|
Exposure 1:
900 MHz
Modulation type:
CW
Exposure duration:
continuous for 5 min., 10 min. or 20 min.
|
|
Exposure 2:
900 MHz
Modulation type:
AM
Exposure duration:
continuous for 5 min., 10 min. or 20 min.
|
|
Frequency | 900 MHz |
---|---|
Type | |
Exposure duration | continuous for 5 min., 10 min. or 20 min. |
Modulation type | CW |
---|
Exposure source | |
---|---|
Distance between exposed object and exposure source | 60 cm |
Setup | double horn antenna placed above the samples |
Sham exposure | A sham exposure was conducted. |
Measurand | Value | Type | Method | Mass | Remarks |
---|---|---|---|---|---|
electric field strength | 10 V/m | effective value | measured | - | - |
power density | 0.26 W/m² | - | - | - | - |
Frequency | 900 MHz |
---|---|
Type | |
Exposure duration | continuous for 5 min., 10 min. or 20 min. |
Modulation type | AM |
---|---|
Modulation frequency | 50 Hz |
Modulation depth | 100 % |
Exposure source |
|
---|---|
Distance between exposed object and exposure source | 60 cm |
Setup | double horn antenna placed above the samples |
Sham exposure | A sham exposure was conducted. |
Measurand | Value | Type | Method | Mass | Remarks |
---|---|---|---|---|---|
electric field strength | 10 V/m | effective value | measured | - | - |
power density | 0.26 W/m² | - | - | - | - |
Significant increases in reactive oxygen species levels and DNA fragmentation were found only in the astrocytes exposed to the modulated electromagnetic field for 20 minutes. No effects were detected when shorter exposure durations or continuous wave exposures were used. The exposure conditions allowed the exclusion of any possible thermal effect.
The data didn't show any significant changes in the release of lactate dehydrogenase, neither for exposure to modulated nor to not modulated (CW) electromagnetic fields.
The data demonstrated, that acute exposure to low intensity microwave electromagnetic fields induces reactive oxygen species production and DNA fragmentation in astrocytes in primary cultures.
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