Medical/biological study (experimental study)

ELF Magnetic Field Effects On Some Hematological And Biochemical Parameters Of Peripheral Blood In Mice med./bio.

By: Cabrales LB, Ciria HC, Bruzon RP, Quevedo MS, Cespedes MC, Salas MF

Published in: Electro Magnetobiol 2001; 20 (2): 185-191

Aim of study (acc. to author)

The effects of a chronic exposure of mice to a 60 Hz magnetic field on hematologic parameters should be investigated.

Background/further details

The mice were split into the following groups (n=20 each): exposure to the magnetic field for 1) 1 month, 2) 6 months, 3) 8 months and 4) a control group (remark EMF-Portal: it is not stated when the control group was investigated). Further remark EMF-Portal: In the discussion, it is stated that 10 mice died after 6 months, although the histological analysis after 8 months was conducted with all rats.

Endpoint

hematologic parameters in mice

Exposure

- 60 Hz
- 50/60 Hz
- magnetic field

Exposure	Parameters
Exposure 1: 60 Hz Exposure duration: continuous (?) for 1 month, 6 months or 8 months	magnetic flux density: 110 µT

Exposure 1

Main characteristics

Frequency	60 Hz
Type	magnetic field
Waveform	sinusoidal
Exposure duration	continuous (?) for 1 month, 6 months or 8 months

Exposure setup

Exposure source	Helmholtz coils
Chamber	48 cm x 24 cm x 14 cm plastic cages
Setup	exposure system consisted of two parallel horizontal flat circular coils (0.65 m radius, 0.65 m distance) with a common axis; MF was perpendicular to the N-S static geomagnetic field; temperature was 23 ± 2°C and relative humidity was 65%

Parameters

Measurand	Value	Type	Method	Mass	Remarks
magnetic flux density	110 µT	-	measured	-	-

Additional parameter details

the static geomagnetic field was 48 µT

Measurement and calculation details

measuring accuracy for magnetic field was ± 100 µT

Exposed system:

animal
mouse/Balb/c
whole body

Methods Endpoint/measurement parameters/methodology

hematologic parameters: hematocrit (centrifugation), hemoglobin level (cyanometahemoglobin method), morphology of erythrocytes, count of leukocytes, neutrophil granulocytes, lymphocytes, monocytes (May-Grünwald-Giemsa stain, haemocytometer, microscopy) (with n=10 mice; only after 1 and 6 months); biochemical parameters: amount of chloride (volumetric method), sodium and potassium (flame photometry), calcium (alizarin method), total protein (biuret method) and glucose (glucosidase method) (with n=10 mice) in peripheral blood; weight of heart, lung, liver, spleen, kidney and brain (with n=20 mice)

Investigated system:

isolated bio./chem. substance
isolated organ
heart, lung, liver, spleen, kidney and brain, blood samples

Time of investigation:

after exposure

Main outcome of study (acc. to author)

In the hematologic parameters, the hematocrit, hemoglobin, neutrophils and leukocytes levels were significantly decreased and the lymphocytes level significantly increased in the exposure group compared to the control group after 6 months.
In the biochemical parameters, the total protein content was significantly decreased after 6 months in the exposure group compared to the control group. After 8 months, the chloride level was significantly increased and the levels of calcium, glucose and total protein were significantly decreased in exposed animals compared to the control group.
The weights of liver and spleen of exposed animals were significantly higher in all exposure groups compared to the control group.
The authors conclude that a chronic exposure of mice to a 60 Hz magnetic field could influence some hematologic parameters and the weight of liver and spleen.

Study character:

medical/biological study
experimental study
full/main study

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